Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Brk/PTK6 antibody [EPR21051-96] - BSA and Azide free (ab234102)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21051-96] to Brk/PTK6 - BSA and Azide free
  • Suitable for: IHC-P, WB, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Brk/PTK6 antibody [EPR21051-96] - BSA and Azide free
    See all Brk/PTK6 primary antibodies

  • Description

    Rabbit monoclonal [EPR21051-96] to Brk/PTK6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon tissue.

  • General notes

    ab234102 is the carrier-free version of ab233392 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab234102 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Brk

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-Brk/PTK6 antibody [EPR21051-96] - BSA and Azide free (ab234102)
    Flow Cytometry - Anti-Brk/PTK6 antibody [EPR21051-96] - BSA and Azide free (ab234102)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized T-47D (human ductal breast epithelial tumor cell line) cell line labeling Brk/PTK6 with ab233392 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233392).

  • Immunoprecipitation - Anti-Brk/PTK6 antibody [EPR21051-96] - BSA and Azide free (ab234102)
    Immunoprecipitation - Anti-Brk/PTK6 antibody [EPR21051-96] - BSA and Azide free (ab234102)

    Brk/PTK6 was immunoprecipitated from 0.35 mg of T-47D (human ductal breast epithelial tumor cell line) whole cell lysate with ab233392 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233392 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: T-47D whole cell lysate 10 µg (Input). 

    Lane 2: ab233392 IP in T-47D whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233392 in T-47D whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    IgG heavy chain: PMID: 18781181

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233392).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brk/PTK6 antibody [EPR21051-96] - BSA and Azide free (ab234102)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brk/PTK6 antibody [EPR21051-96] - BSA and Azide free (ab234102)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Brk/PTK6 with ab233392 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in and membranous staining on human breast cancer cells is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233392).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brk/PTK6 antibody [EPR21051-96] - BSA and Azide free (ab234102)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brk/PTK6 antibody [EPR21051-96] - BSA and Azide free (ab234102)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Brk/PTK6 with ab233392 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in surface epithelium of human colon (PMID: 10430081) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233392).

  • Anti-Brk/PTK6 antibody [EPR21051-96] - BSA and Azide free (ab234102)
    Anti-Brk/PTK6 antibody [EPR21051-96] - BSA and Azide free (ab234102)

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