Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3996] to LI Cadherin - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free
    See all LI Cadherin primary antibodies

  • Description

    Rabbit monoclonal [EPR3996] to LI Cadherin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human small intestine, Human colon cancer, and HT-29 cell lysates. Human LoVo, small intestine and colon lysates. IHC-P: Human colon and Human colonic adenocarcinoma tissues

  • General notes

    ab247786 is the carrier-free version of ab109190 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247786 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Dissociation constant (KD)

    KD = 2.90 x 10 -12 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR3996

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)

    This data was developed using ab109190, the same antibody clone in a different buffer formulation.

    Immunohistochemical staining of paraffin-embedded Human colon tissue using ab109190 at a dilution of 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    Western blot - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    All lanes : Anti-LI Cadherin antibody [EPR3996] (ab109190) at 1/1000 dilution

    Lane 1 : Human small intestine cell lysate
    Lane 2 : Human colon cancer cell lysate
    Lane 3 : HT-29 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 92 kDa



    This data was developed using ab109190, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)

    This data was developed using ab109190, the same antibody clone in a different buffer formulation.

    Immunohistochemical staining of paraffin-embedded Human colonic adenocarcinoma tissue using ab109190 at a dilution of 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)

    This data was developed using ab109190, the same antibody clone in a different buffer formulation.

    ab109190 showing negative staining in Skeletal muscle tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    Western blot - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    All lanes : Anti-LI Cadherin antibody [EPR3996] (ab109190) at 1/1000 dilution (purified)

    Lane 1 : LoVo (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Human small intestine lysate
    Lane 3 : Human colon lysate
    Lane 4 : Human spleen lysate
    Lane 5 : Human kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 92 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab109190, the same antibody clone in a different buffer formulation.

    The expression profile and molecular weight observed are consistent with what has been described in the literature (PMID: 18552820).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    This data was developed using ab109190, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling LI Cadherin with purified ab109190 at 1/300 dilution (0.48 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    This data was developed using ab109190, the same antibody clone in a different buffer formulation.ab109190 showing negative staining in Normal kidney tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    This data was developed using ab109190, the same antibody clone in a different buffer formulation.ab109190 showing negative staining in Normal tonsil tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    This data was developed using ab109190, the same antibody clone in a different buffer formulation.ab109190 showing positive staining in Normal colon tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    This data was developed using ab109190, the same antibody clone in a different buffer formulation.ab109190 showing negative staining in Normal brain tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    OI-RD Scanning - Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    This data was developed using ab109190, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)
    Anti-LI Cadherin antibody [EPR3996] - BSA and Azide free (ab247786)

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