Human P-cadherin (CDH3) Antibody Pair - BSA and Azide free (CDH3) (ab253417)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human P-cadherin (CDH3) Antibody Pair - BSA and Azide free (CDH3)
See all P cadherin kits -
Assay type
ELISA set -
Range
234.375 pg/ml - 15000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human P-cadherin. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab274395), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human P-cadherin (CDH3) Capture Antibody (unconjugated) 1 x 100µg Human P-cadherin (CDH3) Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. -
Tissue specificity
Expressed in some normal epithelial tissues and in some carcinoma cell lines. -
Involvement in disease
Defects in CDH3 are the cause of hypotrichosis with juvenile macular dystrophy (HJMD) [MIM:601553]. HJMD is a rare autosomal recessive disorder characterized by early hair loss heralding severe degenerative changes of the retinal macula and culminating in blindness during the second to third decade of life.
Defects in CDH3 are the cause of ectodermal dysplasia with ectrodactyly and macular dystrophy (EEM) [MIM:225280]; also known as EEM syndrome, Albrectsen-Svendsen syndrome or Ohdo-Hirayama-Terawaki syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EEM is an autosomal recessive condition characterized by features of ectodermal dysplasia such as sparse eyebrows and scalp hair, and selective tooth agenesis associated with macular dystrophy and ectrodactyly. -
Sequence similarities
Contains 5 cadherin domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- CADH3_HUMAN
- Cadherin 3
- Cadherin 3 precursor
see all -
Database links
- Entrez Gene: 1001 Human
- Omim: 114021 Human
- SwissProt: P22223 Human
- Unigene: 191842 Human
Images
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Sandwich ELISA - Human P-cadherin (CDH3) Antibody Pair - BSA and Azide free (CDH3) (ab253417)To learn more about the advantages of recombinant antibodies see here.
