Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Procollagen Type 1 antibody [M-58] (ab64409)

Key features and details

  • Rat monoclonal [M-58] to Procollagen Type 1
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Procollagen Type 1 antibody [M-58]
    See all Procollagen Type 1 primary antibodies

  • Description

    Rat monoclonal [M-58] to Procollagen Type 1

  • Host species

    Rat

  • Specificity

    ab64409 recognizes Human procollagen I. Labels the amino-terminal pro-peptide. Localized intracellularly in cells producing pro-collagen I. Does not stain mature collagen fibers in tissue. The clone number has been updated from (2Q576) to (M-58) both clone numbers name the same antibody clone.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Full length native protein (purified) corresponding to Human Procollagen Type 1.

  • Positive control

    • Human skin

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    Constituent: Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    M-58

  • Isotype

    IgG1

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procollagen Type 1 antibody [M-58] (ab64409)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procollagen Type 1 antibody [M-58] (ab64409)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human skin tissue sections labeling Procollagen Type 1 with ab64409.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procollagen Type 1 antibody [M-58] (ab64409)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procollagen Type 1 antibody [M-58] (ab64409) This image is courtesy of an Abreview submitted by Ross Benik
    ab64409 staining Procollagen Type 1 in Human skin tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with 0.25% serum for 15 minutes at 25°C ; enzymatic antigen retrieval using Proteinase K was performed. Samples were incubated with primary antibody (1/800 in serum free antibody diluent) for 30 minutes at 25°C. A Biotin-conjugated Rabbit anti-rat IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procollagen Type 1 antibody [M-58] (ab64409)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procollagen Type 1 antibody [M-58] (ab64409) This image is courtesy of an anonymous Abreview

    ab64409 staining Procollagen Type 1 (green) in human cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rat IgG polyclonal (1/100) was used as the secondary antibody. Red - Immune Marker, Blue - DAPI.

    See Abreview

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