Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BOP1 antibody [6H11] (ab252819)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [6H11] to BOP1
  • Suitable for: WB, ICC, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-BOP1 antibody [6H11]
    See all BOP1 primary antibodies

  • Description

    Rat monoclonal [6H11] to BOP1

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Caco-2, HeLa and K562 whole cell lysate. ICC; HeLa and K562 cells. IP: K562 whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    6H11

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-BOP1 antibody [6H11] (ab252819)
    Western blot - Anti-BOP1 antibody [6H11] (ab252819)
    All lanes : Anti-BOP1 antibody [6H11] (ab252819) at 1/5000 dilution

    Lane 1 : Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 84 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 10 seconds; Lane 2: 59 seconds; Lane 3: 6 seconds.

  • Immunocytochemistry - Anti-BOP1 antibody [6H11] (ab252819)
    Immunocytochemistry - Anti-BOP1 antibody [6H11] (ab252819)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling BOP1 with ab252819 at 1/50 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue). 

    Negative control 1: ab252819 at a 1/50 dilution followed by ab150080 at a 1/1000 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

  • Immunoprecipitation - Anti-BOP1 antibody [6H11] (ab252819)
    Immunoprecipitation - Anti-BOP1 antibody [6H11] (ab252819)

    BOP1 was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab252819 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab252819 at 1/1,000 dilution. Goat Anti-Rat IgG (H+L), HRP (ab205720) was used at 1/10,000 dilution.

    Lane 1: K562 whole cell lysate 10 µg.

    Lane 2: ab252819 IP in K562 whole cell lysate.

    Lane 3: Rat IgG1 (ab18407) instead of ab252819 in K562 whole cell lysate.

    Blocking and dilution buffer and concentration/ 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Immunocytochemistry - Anti-BOP1 antibody [6H11] (ab252819)
    Immunocytochemistry - Anti-BOP1 antibody [6H11] (ab252819)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labelling BOP1 with ab252819 at 1/50 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in K562 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue). 

    Negative control 1: ab252819 at a 1/50 dilution followed by ab150080 at a 1/1000 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

  • Anti-BOP1 antibody [6H11] (ab252819)
    Anti-BOP1 antibody [6H11] (ab252819)

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