Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TBR1 antibody [EPR8138(2)] (ab183032)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8138(2)] to TBR1
  • Suitable for: ICC/IF, WB, IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TBR1 antibody [EPR8138(2)]
    See all TBR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8138(2)] to TBR1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain lysate. ICC/IF: Mouse Neuron Cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR8138(2)

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-TBR1 antibody [EPR8138(2)] (ab183032)
    Immunocytochemistry/ Immunofluorescence - Anti-TBR1 antibody [EPR8138(2)] (ab183032)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling tbr1 with ab183032 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 antibody [HM-2] at 1/100 dilution and ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594 at 1/1000 (2µg/ml) was used as a MAP2 counterstain. The Nuclear counterstain was DAPI (Blue).

  • Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] (ab183032)
    Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] (ab183032)

    ab183032 staining TBR-1 in Rat E14 cerebral cortex sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with  4% paraformaldehyde and permeabilized with 0.2% TritonX-100.  AlexaFluor®488 Goat anti-Rabbit (2 μg/ml) (ab150077) was used as the secondary antibody. Counter stained using DAPI. Nuclear staining on cerebral cortex of rat E14 [PMID: 27848932].

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody [EPR8138(2)] (ab183032)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody [EPR8138(2)] (ab183032)

    Immunohistochemistry of paraffin embedded Rat E14 cerebral cortex labelling TBR1 with ab183032 at 0.011μg/ml.  Antigen retrieval was heat mediated using Tris/EDTA buffer, pH 9.0 (ab93684). Secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP). Counter stained using Hematoxylin. Nuclear staining on cerebral cortex of rat E14 [PMID: 27848932].

  • Western blot - Anti-TBR1 antibody [EPR8138(2)] (ab183032)
    Western blot - Anti-TBR1 antibody [EPR8138(2)] (ab183032)
    Anti-TBR1 antibody [EPR8138(2)] (ab183032) at 0.9 µg/ml + Human brain lysate at 10 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution

    Predicted band size: 74 kDa


    Exposure time: 30 seconds


    The blocking and dilution buffer used was 5% NFDM/TBST

  • Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] (ab183032)
    Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] (ab183032)

    ab183032 staining TBR-1 in Mouse E14 cerebral cortex sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with  4% paraformaldehyde and permeabilized with 0.2% TritonX-100.  AlexaFluor®488 Goat anti-Rabbit (2 μg/ml) (ab150077) was used as the secondary antibody. Counter stained using DAPI. Nuclear staining on cerebral cortex of mouse E14 [PMID: 27848932].

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody [EPR8138(2)] (ab183032)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody [EPR8138(2)] (ab183032)

    Immunohistochemistry of paraffin embedded Mouse E14 cerebral cortex labelling TBR1 with ab183032 at 0.011μg/ml.  Antigen retrieval was heat mediated using Tris/EDTA buffer, pH 9.0 (ab93684). Secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP). Counter stained using Hematoxylin. Nuclear staining on cerebral cortex of mouse E14 [PMID: 27848932].

     

  • Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] (ab183032)
    Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] (ab183032) This image is courtesy of an Abreview submitted by Daniel Berg.

    Ab183032 staining TBR1 in Human cerebral organoid tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeabilized with 0.1% Triton X, blocked with 5% serum for 1 hour at room temperature and antigen retrieval was by heat mediation in Dako target retrieval. The sample was incubated with primary antibody (1:300 dilution) at 4oC for 12 hours.  An Alexa Fluor® 488 conjugate goat polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-TBR1 antibody [EPR8138(2)] (ab183032)
    Western blot - Anti-TBR1 antibody [EPR8138(2)] (ab183032)
    All lanes : Anti-TBR1 antibody [EPR8138(2)] (ab183032) at 0.9 µg/ml

    Lane 1 : Mouse P1 Cerebral cortex lysate.
    Lane 2 : Mouse hippocampus lysate
    Lane 3 : E18 rat brain lysate
    Lane 4 : Rat P5 cerebral cortex lysate
    Lane 5 : Mouse brain lysate
    Lane 6 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 74 kDa



    The blocking and dilution buffer used was 5% NFDM/TBST.

    Exposure time
    Lane 1 and 2: 10 seconds;
    Lane 3: 5 seconds;
    Lane 4: 2 seconds;
    Lane 5: 3 minutes;
    Lane 6: 15 seconds

  • Western blot - Anti-TBR1 antibody [EPR8138(2)] (ab183032)
    Western blot - Anti-TBR1 antibody [EPR8138(2)] (ab183032)
    Anti-TBR1 antibody [EPR8138(2)] (ab183032) at 1/10000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 74 kDa

  • Anti-TBR1 antibody [EPR8138(2)] (ab183032)
    Anti-TBR1 antibody [EPR8138(2)] (ab183032)

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