Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (ab239000)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8138(2)] to TBR1 - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free
    See all TBR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8138(2)] to TBR1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Fr, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Rat E14 cerebral cortex tissue. ICC/IF: Mouse Neuron Cells.

  • General notes

    ab239000 is the carrier-free version of ab183032.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (ab239000)
    Immunocytochemistry/ Immunofluorescence - Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (ab239000)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling tbr1 with ab183032 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 antibody [HM-2] at 1/100 dilution and ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594 at 1/1000 (2µg/ml) was used as a MAP2 counterstain. The Nuclear counterstain was DAPI (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183032).

  • Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (ab239000)
    Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (ab239000) This image is courtesy of an Abreview submitted by Daniel Berg.

    Ab183032 staining TBR1 in Human cerebral organoid tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeabilized with 0.1% Triton X, blocked with 5% serum for 1 hour at room temperature and antigen retrieval was by heat mediation in Dako target retrieval. The sample was incubated with primary antibody (1:300 dilution) at 4oC for 12 hours.  An Alexa Fluor® 488 conjugate goat polyclonal (1/200) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183032).

  • Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (ab239000)
    Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (ab239000)

    ab183032 staining TBR-1 in Rat E14 cerebral cortex sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with  4% paraformaldehyde and permeabilized with 0.2% TritonX-100.  AlexaFluor®488 Goat anti-Rabbit (2 μg/ml) (ab150077) was used as the secondary antibody. Counter stained using DAPI. Nuclear staining on cerebral cortex of rat E14 [PMID: 27848932].

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183032).

  • Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (ab239000)
    Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (ab239000)

    ab183032 staining TBR-1 in Mouse E14 cerebral cortex sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with  4% paraformaldehyde and permeabilized with 0.2% TritonX-100.  AlexaFluor®488 Goat anti-Rabbit (2 μg/ml) (ab150077) was used as the secondary antibody. Counter stained using DAPI. Nuclear staining on cerebral cortex of mouse E14 [PMID: 27848932].

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (ab239000)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (ab239000)

    Immunohistochemistry of paraffin embedded Mouse E14 cerebral cortex labelling TBR1 with ab183032 at 0.011μg/ml.  Antigen retrieval was heat mediated using Tris/EDTA buffer, pH 9.0 (ab93684). Secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP). Counter stained using Hematoxylin. Nuclear staining on cerebral cortex of mouse E14 [PMID: 27848932].

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (ab239000)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (ab239000)

    Immunohistochemistry of paraffin embedded Rat E14 cerebral cortex labelling TBR1 with ab183032 at 0.011μg/ml.  Antigen retrieval was heat mediated using Tris/EDTA buffer, pH 9.0 (ab93684). Secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP). Counter stained using Hematoxylin. Nuclear staining on cerebral cortex of rat E14 [PMID: 27848932].

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183032).

  • Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (ab239000)
    Anti-TBR1 antibody [EPR8138(2)] - BSA and Azide free (ab239000)

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