Anti-14-3-3 beta antibody (ab15260)
Key features and details
- Rabbit polyclonal to 14-3-3 beta
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-14-3-3 beta antibody
See all 14-3-3 beta primary antibodies -
Description
Rabbit polyclonal to 14-3-3 beta -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human 14-3-3 beta aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P31946 -
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-14-3-3 beta antibody (ab15260)Anti-14-3-3 beta antibody (ab15260) at 1/25 dilution + HeLa cell lysate
Predicted band size: 28 kDa
Observed band size: 28 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 beta antibody (ab15260)ab15260 staining 14-3-3 beta in human brain by Immunohistochemistry (FFPE-sections).
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Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 beta antibody (ab15260)ICC/IF image of ab15260 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15260, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
