Anti-SRGAP1 antibody (ab76926)
Key features and details
- Rabbit polyclonal to SRGAP1
- Suitable for: WB, IP
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-SRGAP1 antibody
See all SRGAP1 primary antibodies -
Description
Rabbit polyclonal to SRGAP1 -
Host species
Rabbit -
Specificity
Does not crossreact with human srGAP2 or human srGAP3 -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human, African green monkey
Predicted to work with: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Bat, Elephant
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Immunogen
Synthetic peptide corresponding to a region between residue 979 and 1029 of human SRGAP1 (NP_065813.1)
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Positive control
- HeLa and 293T whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab76926 was affinity purified using an epitope specific to SRGAP1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SRGAP1 antibody (ab76926) Image courtesy of Alain Chedotal (Institut Vision, France)All lanes : Anti-SRGAP1 antibody (ab76926) at 1/2000 dilution
Lane 1 : COS7 Whole cell lysate
Lane 2 : HEK293H Whole cell lysate
Lane 3 : HEK293H Whole cell lysate overexpressing human srGAP1
Lane 4 : HEK293H whole cell lysate overexpressing human srGAP2
Lane 5 : HEK293H whole cell lysate overexpressing human srGAP3
Lane 6 : HeLa whole cell lysate overexpressing mouse srGAP2
Lane 7 : Mouse E14 hindbrain lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG Conjugated to HRP at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 124 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 170 kDa (possible tagged protein)
Exposure time: 5 minutesBlocking: 5% Milk
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Western blot - Anti-SRGAP1 antibody (ab76926)All lanes : Anti-SRGAP1 antibody (ab76926) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate. at 50 µg
Lane 2 : HeLa whole cell lysate. at 15 µg
Lane 3 : HeLa whole cell lysate. at 5 µg
Lane 4 : 293T whole cell lysate. at 50 µg
Predicted band size: 124 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
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Immunoprecipitation - Anti-SRGAP1 antibody (ab76926)ab76926 at 1µg/ml staining SRGAP1 in HeLa whole cell lysate immunoprecipitated using ab76926 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
