Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

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Anti-KLC1 antibody [EPR12441(B)] (ab174273)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12441(B)] to KLC1
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-KLC1 antibody [EPR12441(B)]
See all KLC1 primary antibodies
Description

Rabbit monoclonal [EPR12441(B)] to KLC1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human KLC1 aa 500 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q07866
Positive control
- U87-MG, 293T, SH-SY5Y, and HeLa whole cell lysate (ab150035); Human brain tissue; U87-MG and HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12441(B)
Isotype

IgG
Research areas

Western blot - Anti-KLC1 antibody [EPR12441(B)] (ab174273)

All lanes : Anti-KLC1 antibody [EPR12441(B)] (ab174273) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : KLC1 knockout HeLa cell lysate
Lane 3 : U-87 MG cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 65 kDa
Observed band size: 65 kDa

Lanes 1-3: Merged signal (red and green). Green - ab174273 observed at 65 kDa. Red - loading control ab8245 observed at 36 kDa.

ab174273 Anti-KLC1 antibody [EPR12441(B)] was shown to specifically react with KLC1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265284 (knockout cell lysate ab257496) was used. Wild-type and KLC1 knockout samples were subjected to SDS-PAGE. ab174273 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (ab174273)

Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling KLC1 with purified ab174273 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

Control: PBS only
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC1 antibody [EPR12441(B)] (ab174273)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling KLC1 with ab174273 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-KLC1 antibody [EPR12441(B)] (ab174273)

All lanes : Anti-KLC1 antibody [EPR12441(B)] (ab174273) at 1/1000 dilution

Lane 1 : U87-MG cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 65 kDa
Immunoprecipitation - Anti-KLC1 antibody [EPR12441(B)] (ab174273)

Western blot analysis on immunoprecipitation pellet from SH-SY5Y cell lysate using ab174273 at 1/10 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (ab174273)

Immunofluorescent analysis of U87-MG cells labeling KLC1 with ab174273 at 1/50 dilution.
Flow Cytometry - Anti-KLC1 antibody [EPR12441(B)] (ab174273)

Flow Cytometric analysis of permeabilized HeLa cells labeling KLC1 with ab174273 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Anti-KLC1 antibody [EPR12441(B)] (ab174273)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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