Anti-KLC2 antibody (ab116702)
Key features and details
- Rabbit polyclonal to KLC2
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-KLC2 antibody
See all KLC2 primary antibodies -
Description
Rabbit polyclonal to KLC2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseWB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following Mouse tissue lysates: Brain; Spinal Cord; Sciatic Nerve; Testis. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse normal testis.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab116702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P MouseWB MouseAll applications RatChinese hamsterApplication Abreviews Notes WB 1/250. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa).IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Notes WB
1/250. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa).IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Target
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Function
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity. -
Sequence similarities
Belongs to the kinesin light chain family.
Contains 6 TPR repeats. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 16594 Mouse
- Entrez Gene: 309159 Rat
- SwissProt: O88448 Mouse
- Unigene: 271648 Mouse
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Alternative names
- Kinesin light chain 2 antibody
- KLC 2 antibody
- KLC2 antibody
- KLC2_HUMAN antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC2 antibody (ab116702)
IHC image of KLC2 staining in Mouse normal testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval (EDTA based pH 9.0 solution) for 20 mins. The section was then incubated with ab116702, 1µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-KLC2 antibody (ab116702)All lanes : Anti-KLC2 antibody (ab116702) at 1/250 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 : Sciatic Nerve (Mouse) Tissue Lysate
Lane 4 : Testis (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab116702 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
Datasheets and documents
References (0)
ab116702 has not yet been referenced specifically in any publications.
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC2 antibody (ab116702)
IHC image of KLC2 staining in Mouse normal testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval (EDTA based pH 9.0 solution) for 20 mins. The section was then incubated with ab116702, 1µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-KLC2 antibody (ab116702)All lanes : Anti-KLC2 antibody (ab116702) at 1/250 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 : Sciatic Nerve (Mouse) Tissue Lysate
Lane 4 : Testis (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab116702 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
