Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KLC2 antibody (ab154212)

Key features and details

  • Rabbit polyclonal to KLC2
  • Suitable for: WB
  • Reacts with: Mouse
  • Isotype: IgG

Overview

  • Product name

    Anti-KLC2 antibody
    See all KLC2 primary antibodies

  • Description

    Rabbit polyclonal to KLC2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Mouse KLC2 aa 100-200 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab154626)

  • Positive control

    • This antibody gave a positive signal in the following Mouse tissue lysates: Brain; Spinal Cord; Sciatic Nerve; Testis.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa).

Target

  • Function

    Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.

  • Sequence similarities

    Belongs to the kinesin light chain family.
    Contains 6 TPR repeats.

  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • Database links

    • Alternative names

      • Kinesin light chain 2 antibody
      • KLC 2 antibody
      • KLC2 antibody
      • KLC2_HUMAN antibody

    Images

    • Western blot - Anti-KLC2 antibody (ab154212)
      Western blot - Anti-KLC2 antibody (ab154212)
      All lanes : Anti-KLC2 antibody (ab154212) at 1 µg/ml

      Lane 1 : Brain (Mouse) Tissue Lysate
      Lane 2 : Spinal Cord (Mouse) Tissue Lysate
      Lane 3 : Sciatic Nerve (Mouse) Tissue Lysate

      Lane 4 : Testis (Mouse) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 66 kDa
      Observed band size: 70 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 103 kDa, 37 kDa, 45 kDa, 99 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 10 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab154212 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    References (0)

    Publishing research using ab154212? Please let us know so that we can cite the reference in this datasheet.

    ab154212 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-KLC2 antibody (ab154212)
      Western blot - Anti-KLC2 antibody (ab154212)
      All lanes : Anti-KLC2 antibody (ab154212) at 1 µg/ml

      Lane 1 : Brain (Mouse) Tissue Lysate
      Lane 2 : Spinal Cord (Mouse) Tissue Lysate
      Lane 3 : Sciatic Nerve (Mouse) Tissue Lysate

      Lane 4 : Testis (Mouse) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 66 kDa
      Observed band size: 70 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 103 kDa, 37 kDa, 45 kDa, 99 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 10 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab154212 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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