Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14838] to MCAK - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MCAK antibody [EPR14838] - BSA and Azide free
    See all MCAK primary antibodies

  • Description

    Rabbit monoclonal [EPR14838] to MCAK - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250931 is the carrier-free version of ab187652.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931)
    Western blot - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931)

    This data was developed using ab187652, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: MCAK knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HeLa whole cell lysate (20 µg)

    Lane 4: HEK293 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab187652 observed at 90 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab187652 was shown to specifically react with MCAK when MCAK knockout samples were used. Wild-type and MCAK knockout samples were subjected to SDS-PAGE. ab187652 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931)
    Flow Cytometry - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931)
    This data was developed using ab187652, the same antibody clone in a different buffer formulation.Flow cytometric analysis of HeLa cells labeling MCAK with ab187652 at 1/260 dilution compared to negative control cells. Secondary antibody FITC-conjugated goat-anti-rabbit IgG used at 1/150 diution.
  • Western blot - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931)
    Western blot - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931)
    All lanes : Anti-MCAK antibody [EPR14838] (ab187652) at 1/1000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 3 : Hela cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 81 kDa



    This data was developed using ab187652, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931)
    Immunocytochemistry - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931)
    This data was developed using ab187652, the same antibody clone in a different buffer formulation.Immunofluorscence analysis of MCF7 cells labeling MCAK with ab187652 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter-stained with Dapi.
  • Immunocytochemistry - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931)
    Immunocytochemistry - Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931)
    This data was developed using ab187652, the same antibody clone in a different buffer formulation.Immunofluorscence analysis of HeLa cells labeling MCAK with ab187652 at 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter-stained with Dapi.
  • Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931)
    Anti-MCAK antibody [EPR14838] - BSA and Azide free (ab250931)

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