Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10740(B)] to Kinesin Heavy Chain 2
Suitable for: WB, IHC-P, ICC/IF
Knockout validated
Reacts with: Human

Product name

Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)]
See all Kinesin Heavy Chain 2 primary antibodies
Description

Rabbit monoclonal [EPR10740(B)] to Kinesin Heavy Chain 2
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to residues in Human Kinesin Heavy Chain 2 (Uniprot ID O00139).
Positive control
- Human fetal brain tissue lysate; Jurkat, 293T cell lysate; Human thyroid carcinoma tissue; Jurkat cells
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10740(B)
Isotype

IgG
Research areas

Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Kinesin Heavy Chain 2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)

Lanes 1 - 3: Merged signal (red and green). Green - ab154868 observed at 100 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab154868 was shown to recognize Kinesin Heavy Chain 2 in wild-type HAP1 cells as signal was lost at the expected MW in Kinesin Heavy Chain 2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Kinesin Heavy Chain 2 knockout samples were subjected to SDS-PAGE. Ab154868 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)

Immunofluorescent analysis of Jurkat cells labeling Kinesin Heavy Chain 2 with ab154868 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)

Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma labeling Kinesin Heavy Chain 2 with ab154868 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)

All lanes : Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868) at 1/1000 dilution

Lane 1 : Human fetal brain tissue lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 187 kDa
Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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