Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KLC3 antibody [EPR11149(2)] (ab180523)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11149(2)] to KLC3
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-KLC3 antibody [EPR11149(2)]
    See all KLC3 primary antibodies

  • Description

    Rabbit monoclonal [EPR11149(2)] to KLC3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human KLC3 aa 350-450. The exact sequence is proprietary.
    Database link: Q6P597

  • Positive control

    • Jurkat, U87-MG, HepG2 and BxPC-3 cell lysates; Human liver tissue; HepG2 and Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11149(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-KLC3 antibody [EPR11149(2)] (ab180523)
    Western blot - Anti-KLC3 antibody [EPR11149(2)] (ab180523)
    All lanes : Anti-KLC3 antibody [EPR11149(2)] (ab180523) at 1/50000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : U87-MG cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : BxPC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 antibody [EPR11149(2)] (ab180523)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 antibody [EPR11149(2)] (ab180523)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling KLC3 with ab180523 at 1/250 dilution followed by ImmunoHistoprobe (Ready to use)  HRP Polymer for Rabbit IgG secondary and counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-KLC3 antibody [EPR11149(2)] (ab180523)
    Immunocytochemistry/ Immunofluorescence - Anti-KLC3 antibody [EPR11149(2)] (ab180523)

    Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling KLC3 with ab180523 at 1/1000 dilution followed by Goat anti-rabbit IgG (Dylight 555) at 1/250 dilution and counterstained with DAPI.

  • Immunoprecipitation - Anti-KLC3 antibody [EPR11149(2)] (ab180523)
    Immunoprecipitation - Anti-KLC3 antibody [EPR11149(2)] (ab180523)

    Western blot analysis of immunoprecipitation pellet from BxPC-3 cell lysate immunoprecipitated using ab180523 at 1/40 dilution (lane 1). Negative control (lane 2). Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1500 dilution was used as secondary.

  • Flow Cytometry - Anti-KLC3 antibody [EPR11149(2)] (ab180523)
    Flow Cytometry - Anti-KLC3 antibody [EPR11149(2)] (ab180523)

    Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling KLC3 with ab180523 at 1/10 dilution. Goat anti-rabbit IgG (FITC) at 1/75 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.

  • Anti-KLC3 antibody [EPR11149(2)] (ab180523)
    Anti-KLC3 antibody [EPR11149(2)] (ab180523)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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