Anti-KLC3 antibody (ab101188)
Key features and details
- Rabbit polyclonal to KLC3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KLC3 antibody
See all KLC3 primary antibodies -
Description
Rabbit polyclonal to KLC3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following tissue lysates: Human spleen; Human small intestine; Human ovary; Human testis.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications Macaque monkeyOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).ICC/IF Use a concentration of 5 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).ICC/IF
Use a concentration of 5 µg/ml.Target
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Function
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. -
Sequence similarities
Belongs to the kinesin light chain family.
Contains 5 TPR repeats. -
Domain
The heptad repeat (HR) motif is sufficient for interaction with kinesin heavy (KHL) chains and ODF1. The TPR region is involved in mitochondrial binding. -
Cellular localization
Cytoplasm > cytoskeleton. Elongating spermatid tail midpiece, localized in outer dense fibers (ODFs) and associates with mitochondria. - Information by UniProt
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Database links
- Entrez Gene: 147700 Human
- Entrez Gene: 100173098 Orangutan
- Omim: 601334 Human
- SwissProt: Q6P597 Human
- SwissProt: Q5R8E2 Orangutan
- Unigene: 298079 Human
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Alternative names
- Kinesin light chain 2 antibody
- Kinesin light chain 3 antibody
- KLC2 antibody
see all
Images
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Western blot - Anti-KLC3 antibody (ab101188)All lanes : Anti-KLC3 antibody (ab101188) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lane 3 : Human ovary tissue lysate - total protein (ab30222)
Lane 4 : Human testis tissue lysate - total protein (ab30257)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Additional bands at: 29 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 14 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-KLC3 antibody (ab101188)ICC/IF image of ab101188 stained U87MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101188, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (0)
ab101188 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-KLC3 antibody (ab101188)All lanes : Anti-KLC3 antibody (ab101188) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lane 3 : Human ovary tissue lysate - total protein (ab30222)
Lane 4 : Human testis tissue lysate - total protein (ab30257)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Additional bands at: 29 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 14 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-KLC3 antibody (ab101188)ICC/IF image of ab101188 stained U87MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101188, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
