Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SA1 antibody [SUSI63B] - BSA and Azide free (ab252782)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [SUSI63B] to SA1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SA1 antibody [SUSI63B] - BSA and Azide free
    See all SA1 primary antibodies

  • Description

    Rat monoclonal [SUSI63B] to SA1 - BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment corresponding to SA1 (N terminal). Immunogen species mouse and human.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Jurkat, MOLT-4, MCF7, A431, TF-1, RAW264.7 and PC-12 whole cell lysate. IHC-P: Human colon and pancreas tissue. Mouse and rat kidney tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab252782 is the carrier-free version of ab241544. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] - BSA and Azide free (ab252782)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] - BSA and Azide free (ab252782)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling SA1 with ab241544 at 0.765 µg/ml , followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on human colon tissue is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241544).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] - BSA and Azide free (ab252782)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] - BSA and Azide free (ab252782)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling SA1 with ab241544 at 0.765 µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse kidney tissue is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241544).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] - BSA and Azide free (ab252782)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] - BSA and Azide free (ab252782)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling SA1 with ab241544 at 0.765 µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on human pancreas tissue is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241544).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] - BSA and Azide free (ab252782)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody [SUSI63B] - BSA and Azide free (ab252782)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling SA1 with ab241544 at 0.765 µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on rat kidney tissue is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241544).

  • Western blot - Anti-SA1 antibody [SUSI63B] - BSA and Azide free (ab252782)
    Western blot - Anti-SA1 antibody [SUSI63B] - BSA and Azide free (ab252782)
    All lanes : Anti-SA1 antibody [SUSI63B] (ab241544) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
    Lane 5 : TF-1 (human Erythroleukemia erythroblast), whole cell
    Lanes 6-7 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 144 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22715410).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241544).

  • Anti-SA1 antibody [SUSI63B] - BSA and Azide free (ab252782)
    Anti-SA1 antibody [SUSI63B] - BSA and Azide free (ab252782)

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