Human Podocin Antibody Pair - BSA and Azide free (ab256649)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
-
Product name
Human Podocin Antibody Pair - BSA and Azide free
See all NPHS2 kits -
Assay type
ELISA set -
Range
15.63 pg/ml - 1000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human Podocin. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab245708), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
-
Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human Podocin Capture Antibody (unconjugated) 1 x 100µg Human Podocin Detector Antibody (unconjugated) 1 x 100µg -
Research areas
-
Function
Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. -
Tissue specificity
Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli. -
Involvement in disease
Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2) [MIM:600995]. It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. -
Sequence similarities
Belongs to the band 7/mec-2 family. -
Cellular localization
Cell membrane. - Information by UniProt
-
Alternative names
- nephrosis 2
- nephrosis 2, idiopathic, steroid resistant
- Nephrosis 2, idiopathic, steroid resistant (podocin)
see all -
Database links
- Entrez Gene: 7827 Human
- Omim: 604766 Human
- SwissProt: Q9NP85 Human
- Unigene: 412710 Human
Images
-
Sandwich ELISA - Human Podocin Antibody Pair - BSA and Azide free (ab256649)To learn more about the advantages of recombinant antibodies see here.
