Anti-ORP150 antibody [EPR5890] - BSA and Azide free (ab248038)
![Anti-ORP150 antibody [EPR5890] - BSA and Azide free (ab248038)](https://www.abcam.com/ps/products/248/ab248038/Images/ab248038-408185-anti-orp150-antibody-epr5890-bsa-and-azide-free-western-blot.jpg "Anti-ORP150 antibody [EPR5890] - BSA and Azide free (ab248038)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5890] to ORP150 - BSA and Azide free
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ORP150 antibody [EPR5890] - BSA and Azide free
See all ORP150 primary antibodies -
Description
Rabbit monoclonal [EPR5890] to ORP150 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab156891) -
General notes
Ab248038 is the carrier-free version of ab124884. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248038 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 5.40 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR5890 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ORP150 antibody [EPR5890] - BSA and Azide free (ab248038)All lanes : Anti-ORP150 antibody [EPR5890] (ab124884) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Rat liver tissue lysate
Lane 3 : Rat pancreas tissue lysate
Lane 4 : 293T cell lysate
Lane 5 : MCF-7 cell lysate
Lane 6 : MDA-MB-435S cell lysate
Lane 7 : SH-SY5Y cell lysate
Lane 8 : HeLa cell lysate
Lysates/proteins at 10 µg/ml per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 111 kDaThis data was developed using ab124884, the same antibody clone in a different buffer formulation.
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![Flow Cytometry - Anti-ORP150 antibody [EPR5890] - BSA and Azide free (ab248038)](https://www.abcam.com/ps/products/248/ab248038/Images/ab248038-3-anti-orp150-antibody-epr5890-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-ORP150 antibody [EPR5890] - BSA and Azide free (ab248038)This data was developed using ab124884, the same antibody clone in a different buffer formulation.Overlay histogram showing HepG2 cells stained with ab124884 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124884, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP150 antibody [EPR5890] - BSA and Azide free (ab248038)](https://www.abcam.com/ps/products/248/ab248038/Images/ab248038-2-anti-orp150-antibody-epr5890-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP150 antibody [EPR5890] - BSA and Azide free (ab248038)This data was developed using ab124884, the same antibody clone in a different buffer formulation.ab124884, at 1/250 dilution, staining ORP150 in paraffin-embedded Human liver tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![OI-RD Scanning - Anti-ORP150 antibody [EPR5890] - BSA and Azide free (ab248038)](https://www.abcam.com/ps/products/248/ab248038/Images/ab248038-1-anti-orp150-antibody-epr5890-bsa-and-azide-free-equilibrium-disassociation-constant-kd.jpg)
OI-RD Scanning - Anti-ORP150 antibody [EPR5890] - BSA and Azide free (ab248038)This data was developed using ab124884, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
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![Anti-ORP150 antibody [EPR5890] - BSA and Azide free (ab248038)](https://www.abcam.com/ps/products/248/ab248038/Images/ab248038-5-benefits-of-recombinant-antibodies.png)
Anti-ORP150 antibody [EPR5890] - BSA and Azide free (ab248038)