Anti-S100A4 antibody (ab93283)
Key features and details
- Mouse monoclonal to S100A4
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-S100A4 antibody
See all S100A4 primary antibodies -
Description
Mouse monoclonal to S100A4 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentICC/IF HumanWB MouseHuman
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Immunogen
Recombinant full length protein corresponding to amino acids 1-102 (MACPLEKALD VMVSTFHKYS GKEGDKFKLN KSELKELLTR ELPSFLGKRT) of Human S100A4 (AAH16300) with a proprietary tag.
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Positive control
- HeLa cell and NIH/3T3 lysates. Transfected 293T cell line.
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General notes
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-S100A4 antibody (ab93283)Anti-S100A4 antibody (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDa
Observed band size: 37.22 kDa why is the actual band size different from the predicted?Predicted band size for the immunogen in 37.22 kDa.
This image was generated using the ascites version of the product.
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Western blot - Anti-S100A4 antibody (ab93283)Anti-S100A4 antibody (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + HeLa cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDaThis image was generated using the ascites version of the product.
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Western blot - Anti-S100A4 antibody (ab93283)Anti-S100A4 antibody (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + NIH/3T3 cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDaThis image was generated using the ascites version of the product.
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Western blot - Anti-S100A4 antibody (ab93283)All lanes : Anti-S100A4 antibody (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer)
Lane 1 : cell lysate from S100A4 transfected 293T cell line
Lane 2 : cell lysate from non transfected 293T cell line
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDa
Observed band size: 11.7 kDa why is the actual band size different from the predicted?This image was generated using the ascites version of the product.
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Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody (ab93283)ab93283 at 15µg/ml staining S100A4 in fixed and permeabilised HeLa cells by immunofluorescence.
This image was generated using the ascites version of the product.
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ELISA - Anti-S100A4 antibody (ab93283)Detection limit for recombinant GST tagged S100A4 is 0.1 ng/ml as a capture antibody.
