Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human PAEP ELISA Kit (Glycodelin) (ab275904)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 3 pg/ml
  • Range: 7.81 pg/ml - 500 pg/ml
  • Sample type: Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human PAEP ELISA Kit (Glycodelin)
    See all PAEP/Glycodelin kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 5.2%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 7.98%

  • Sample type

    Serum

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    3 pg/ml

  • Range

    7.81 pg/ml - 500 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 107 89% - 116%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human PAEP ELISA kit (ab275904) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PAEP protein in human serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PAEP with 3 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less


            -High sensitivity, specificity and reproducibility from superior antibodies


            -Fully validated in biological samples


            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    PAEP, progestogen-associated endometrial protein also known as glycodelin, is a glycoprotein which plays many important roles in fertility and maintenance of pregnancy. PAEP changes its biochemical interaction based on its glycosylation state. PAEP is a prognostic marker for in vitro fertilization and for premature membrane rupture. The antibodies in this kit were raised against a recombinant full-length mature protein. This protein does not have a homolog in mouse or rat and is not expected to cross-react with samples from these species.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human PAEP (Glycodelin) Capture Antibody 1 x 600ml
    10X Human PAEP (Glycodelin) Detector Antibody 1 x 600ml
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Human PAEP (Glycodelin) Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    This protein is, quantitatively, the main protein synthesized and secreted in the endometrium from mid-luteal phase of the menstrual cycle and during the first semester of pregnancy.

  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family.

  • Post-translational
    modifications

    At least two differentially glycosylated forms are found. Glycodelin-A (GdA) (amniotic fluid) with contraceptive and immunosuppressive activities and glycodelin-S (Gds) (seminal plasma) whose role is not yet known. The gender-specific glycosylation may serve to regulate key process involved in human reproduction.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Alpha uterine protein
    • gD
    • GdA
    • GdF
    • GdS
    • Glycodelin
    • Glycodelin A
    • Glycodelin F
    • Glycodelin S
    • MGC138509
    • MGC142288
    • PAEG
    • PAEP
    • PAEP_HUMAN
    • PEG
    • PEP
    • Placental protein 14
    • Placental Protein 14 / Glycodelin A
    • PP14
    • Pregnancy-associated endometrial alpha-2 globulin
    • Progestagen-associated endometrial protein
    • Progesterone-associated endometrial protein
    see all

  • Database links

    Images

    • SimpleStep ELISA Protocol Diagram
      SimpleStep ELISA Protocol Diagram

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

    • Example of Human PAEP standard curve in Sample Diluent NS.
      Example of Human PAEP standard curve in Sample Diluent NS.
    • Interpolated concentrations of native PAEP in Human serum samples.
      Interpolated concentrations of native PAEP in Human serum samples.
    • Sandwich ELISA - Human PAEP ELISA Kit (Glycodelin) (ab275904)
      Sandwich ELISA - Human PAEP ELISA Kit (Glycodelin) (ab275904)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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