Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HABP2 antibody [EPR14551] (ab181837)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14551] to HABP2
  • Suitable for: WB, Flow Cyt, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HABP2 antibody [EPR14551]
    See all HABP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14551] to HABP2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: MCF7. Flow Cyt: HepG2. WB: MCF2 and A549 whole cell lysate. Mouse brain and heart lysate. Rate brain and heart lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14551

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-HABP2 antibody [EPR14551] (ab181837)
    Western blot - Anti-HABP2 antibody [EPR14551] (ab181837)
    Lane 1 : Anti-HABP2 antibody [EPR14551] (ab181837) at 1/10000 dilution (Purified)
    Lanes 2-6 : Anti-HABP2 antibody [EPR14551] (ab181837) at 1/10000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
    Lane 2 : A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg
    Lane 3 : Mouse brain lysate at 20 µg
    Lane 4 : Mouse heart lysate at 20 µg
    Lane 5 : Rat brain lysate at 1/20 dilution
    Lane 6 : Rat heart lysate at 20 µg

    Secondary
    Lanes 1-2 & 4-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
    Lane 3 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
    Lane 6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg

    Predicted band size: 63 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry - Anti-HABP2 antibody [EPR14551] (ab181837)
    Immunocytochemistry - Anti-HABP2 antibody [EPR14551] (ab181837)
    Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling HABP2 with purified ab181837 at 1/50 dilution (2.46 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry - Anti-HABP2 antibody [EPR14551] (ab181837)
    Flow Cytometry - Anti-HABP2 antibody [EPR14551] (ab181837)
    Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling HABP2 with purified ab181837 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Anti-HABP2 antibody [EPR14551] (ab181837)
    Anti-HABP2 antibody [EPR14551] (ab181837)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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