Human Serpin A5 ELISA Kit (ab277462)
Key features and details
- Sensitivity: 24 pg/ml
- Range: 24.58 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human Serpin A5 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
24 pg/ml -
Range
24.58 pg/ml - 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85.61 80% - 89% Plasma 87.32 83% - 93% Cell culture media 72.11 67% - 80% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Serpin A5 ELISA Kit (ab277462) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Serpin A5 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Serpin A5 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Serpin A5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Serpin A5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Serpin A5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human Serpin A5 Antibody 2 vials Anti-Human Serpin A5 coated Microplate 1 unit Human Serpin A5 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Function
Inhibits activated protein C as well as plasminogen activators. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Sequence similarities
Belongs to the serpin family. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- Acrosomal serine protease inhibitor
- IPSP
- IPSP_HUMAN
see all -
Database links
- Entrez Gene: 5104 Human
- Omim: 601841 Human
- SwissProt: P05154 Human
- Unigene: 159628 Human
Images
-
Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.