Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S381) antibody [EPR1887] - BSA and Azide free (ab247874)
![Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S381) antibody [EPR1887] - BSA and Azide free (ab247874)](https://www.abcam.com/ps/products/247/ab247874/Images/ab247874-408151-anti-glutamate-receptor-1-ampa-subtype-phospho-s381-antibody-epr1887-bsa-and-azide-free-western-blot.jpg "Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S381) antibody [EPR1887] - BSA and Azide free (ab247874)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1887] to Glutamate Receptor 1 (AMPA subtype) (phospho S381) - BSA and Azide free
- Suitable for: Dot blot, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S381) antibody [EPR1887] - BSA and Azide free
See all Glutamate Receptor 1 (AMPA subtype) primary antibodies -
Description
Rabbit monoclonal [EPR1887] to Glutamate Receptor 1 (AMPA subtype) (phospho S381) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody only detects Glutamate Receptor 1 phosphorylated at Serine 831.
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Tested applications
Suitable for: Dot blot, WBmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247874 is the carrier-free version of ab109464. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247874 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR1887 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S381) antibody [EPR1887] - BSA and Azide free (ab247874)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution
Lane 1 : Mouse hippocampus whole cell lysate
Lane 2 : Mouse hippocampus whole cell lysate, the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsThis data was developed using ab109464, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S381) antibody [EPR1887] - BSA and Azide free (ab247874)](https://www.abcam.com/ps/products/247/ab247874/Images/ab247874-3-anti-glutamate-receptor-1-ampa-subtype-phospho-s381-antibody-epr1887-bsa-and-azide-free-dot-blot.jpg)
Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S381) antibody [EPR1887] - BSA and Azide free (ab247874)This data was developed using ab109464, the same antibody clone in a different buffer formulation.Dot blot analysis of Glutamate Receptor 1 (AMPA subtype) with ab109464 at 1/1000 exposed for 3 minutes. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) (1/100,000) was used as the secondary antibody. Blocking buffer 5% NFDM/TBST. Diluting buffer 5% NFDM/TBST. Lane 1: Glutamate Receptor 1 (AMPA subtype) (pS831) phospho peptide
Lane 2: Glutamate Receptor 1 (AMPA subtype) non-phospho peptide -
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S381) antibody [EPR1887] - BSA and Azide free (ab247874)](https://www.abcam.com/ps/products/247/ab247874/Images/ab247874-403257-anti-glutamate-receptor-1-ampa-subtype-phospho-s381-antibody-epr1887-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S381) antibody [EPR1887] - BSA and Azide free (ab247874)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution
Lane 1 : Human cerebellum whole cell lysates
Lane 2 : Human cerebellum whole cell lysates, the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis data was developed using ab109464, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S381) antibody [EPR1887] - BSA and Azide free (ab247874)](https://www.abcam.com/ps/products/247/ab247874/Images/ab247874-398907-anti-glutamate-receptor-1-ampa-subtype-phospho-s381-antibody-epr1887-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S381) antibody [EPR1887] - BSA and Azide free (ab247874)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S831) antibody [EPR1887] (ab109464) at 1/1000 dilution
Lane 1 : Rat brain lysates, untreated
Lane 2 : Rat brain lysates treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 106 kDa why is the actual band size different from the predicted?This data was developed using ab109464, the same antibody clone in a different buffer formulation.
The lower panel shows Glutamate Receptor 1 detected with an alternative anti-Glutamate Receptor 1 antibody which is not dependent upon antigen phosphorylation.
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![Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S381) antibody [EPR1887] - BSA and Azide free (ab247874)](https://www.abcam.com/ps/products/247/ab247874/Images/ab247874-5-benefits-of-recombinant-antibodies.png)
Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S381) antibody [EPR1887] - BSA and Azide free (ab247874)
