Anti-NCF1/p47-phox antibody [EPR13134-23] - BSA and Azide free (ab250338)
![Anti-NCF1/p47-phox antibody [EPR13134-23] - BSA and Azide free (ab250338)](https://www.abcam.com/ps/products/250/ab250338/Images/ab250338-405805-anti-ncf1-p47-phox-antibody-epr13134-23-bsa-and-azide-free-western-blot.jpg "Anti-NCF1/p47-phox antibody [EPR13134-23] - BSA and Azide free (ab250338)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13134-23] to NCF1/p47-phox - BSA and Azide free
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
Overview
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Product name
Anti-NCF1/p47-phox antibody [EPR13134-23] - BSA and Azide free
See all NCF1/p47-phox primary antibodies -
Description
Rabbit monoclonal [EPR13134-23] to NCF1/p47-phox - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250338 is the carrier-free version of ab181090. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250338 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR13134-23 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NCF1/p47-phox antibody [EPR13134-23] - BSA and Azide free (ab250338)All lanes : Anti-NCF1/p47-phox antibody [EPR13134-23] (ab181090) at 1/2000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Ramos cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDaThis data was developed using ab181090, the same antibody clone in a different buffer formulation.
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![Immunoprecipitation - Anti-NCF1/p47-phox antibody [EPR13134-23] - BSA and Azide free (ab250338)](https://www.abcam.com/ps/products/250/ab250338/Images/ab250338-2-anti-ncf1-p47-phox-antibody-epr13134-23-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-NCF1/p47-phox antibody [EPR13134-23] - BSA and Azide free (ab250338)This data was developed using ab181090, the same antibody clone in a different buffer formulation.Western blot analysis of Raji cell lysate immunoprecipitated with ab181090 at 1/20 dilution (Lane 1). Lane 2: Negative control.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCF1/p47-phox antibody [EPR13134-23] - BSA and Azide free (ab250338)](https://www.abcam.com/ps/products/250/ab250338/Images/ab250338-1-anti-ncf1-p47-phox-antibody-epr13134-23-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCF1/p47-phox antibody [EPR13134-23] - BSA and Azide free (ab250338)This data was developed using ab181090, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling NCF1/p47-phox with ab181090 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Anti-NCF1/p47-phox antibody [EPR13134-23] - BSA and Azide free (ab250338)](https://www.abcam.com/ps/products/250/ab250338/Images/ab250338-4-benefits-of-recombinant-antibodies.png)
Anti-NCF1/p47-phox antibody [EPR13134-23] - BSA and Azide free (ab250338)
