Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1018Y] to Serum Amyloid P/SAP
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-Serum Amyloid P/SAP antibody [EP1018Y]
See all Serum Amyloid P/SAP primary antibodies
Description

Rabbit monoclonal [EP1018Y] to Serum Amyloid P/SAP
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Serum Amyloid P/SAP aa 1 to the C-terminus (N terminal). The exact sequence is proprietary.
Positive control
- WB: Human serum; Human liver lysate. IHC-P: Human endometrial carcinoma tissue.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Serum Amyloid P.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1018Y
Isotype

IgG
Research areas

Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)

Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151) at 1/10000 dilution + Human serum at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 25 kDa

Diluting and blocking buffer: 5% NFDM /TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)

ab45151 staining Serum Amyloid P/SAP in human endometrial carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

Negative control 1: PBS in place of primary antibody.
Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)

Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151) at 1/5000 dilution + Human liver at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 25 kDa

Blocking and diluting buffer: 5% NFDM /TBST
Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com