Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1018Y] to Serum Amyloid P/SAP
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Serum Amyloid P/SAP antibody [EP1018Y]
    See all Serum Amyloid P/SAP primary antibodies

  • Description

    Rabbit monoclonal [EP1018Y] to Serum Amyloid P/SAP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Serum Amyloid P/SAP aa 1 to the C-terminus (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Human serum; Human liver lysate. IHC-P: Human endometrial carcinoma tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as Serum Amyloid P.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)
    Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)
    Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151) at 1/10000 dilution + Human serum at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 25 kDa



    Diluting and blocking buffer: 5% NFDM /TBST 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)

    ab45151 staining Serum Amyloid P/SAP in human endometrial carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)
    Western blot - Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)
    Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151) at 1/5000 dilution + Human liver at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 25 kDa



    Blocking and diluting buffer: 5% NFDM /TBST

  • Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)
    Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Serum Amyloid P/SAP antibody [EP1018Y] (ab45151)

© 2021 Astana Biomed Group LLP. All rights reserved.