Anti-Patched / PTCH1 antibody (ab51983)
Key features and details
- Goat polyclonal to Patched / PTCH1
- Suitable for: ICC, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Patched / PTCH1 antibody
See all Patched / PTCH1 primary antibodies -
Description
Goat polyclonal to Patched / PTCH1 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species ICC MouseWB Human
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Immunogen
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-Patched / PTCH1 antibody (ab51983)Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells permeabilized with 0.15% Triton staining Patched / PTCH1. Primary incubation with ab51983 (5µg/ml) for 1 hour followed by Alexa Fluor 488 secondary antibody (2µg/ml). Nuclear counter stain is DAPI.
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Western blot - Anti-Patched / PTCH1 antibody (ab51983)Anti-Patched / PTCH1 antibody (ab51983) at 1 µg/ml + Human Brain lysate in RIPA buffer at 35 µg
Predicted band size: 161 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
