Anti-Patched / PTCH1 antibody (ab53715)
Key features and details
- Rabbit polyclonal to Patched / PTCH1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Patched / PTCH1 antibody
See all Patched / PTCH1 primary antibodies -
Description
Rabbit polyclonal to Patched / PTCH1 -
Host species
Rabbit -
Specificity
ab53715 detects endogenous levels of total Patched/PTCH1 protein. In WB, in addition to the expected band at 150 kDa an extra band at 75 kDa is often detected. Both bands can be blocked with the immunogen peptide. In IHC, cytoplasmic and membrane staining is frequently observed. These results are similar to what we see with other antibodies against this target. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Patched/ PTCH1 (N terminal). Immunogen is in the range of aa 1-50.
Database link: Q13635 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Patched / PTCH1 antibody (ab53715) at 1/500 dilution
Lane 1 : Extracts from mouse muscle cells, minus immunising peptide
Lane 2 : Extracts from mouse muscle cells, plus immunising peptide
Predicted band size: 161 kDa
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ab53715, at a 1/50 dilution, staining Patched / PTCH in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.