Anti-AMPK alpha 2 antibody (ab3760)
Key features and details
- Rabbit polyclonal to AMPK alpha 2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-AMPK alpha 2 antibody
See all AMPK alpha 2 primary antibodies -
Description
Rabbit polyclonal to AMPK alpha 2 -
Host species
Rabbit -
Specificity
Does not cross-react with AMPK alpha 1. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Guinea pig, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
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Immunogen
Synthetic peptide (Human) - which represented a portion of AMP activated protein kinase, alpha-2 catalytic subunit encoded within exon 7.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AMPK alpha 2 antibody (ab3760)All lanes : Anti-AMPK alpha 2 antibody (ab3760) at 0.4 µg/ml
Lane 1 : HeLa (Human epithelial adenocarcinoma cell line) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : Renca (Mouse renal adenocarcinoma cell line) whole cell lysate
Lane 5 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 63 kDa
Exposure time: 3 minutesChemiluminescence detection.
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Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 2 antibody (ab3760)ICC/IF image of ab3760 stained MCF7 (Human breast adenocarcinoma cell line) cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3760, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
