Anti-IL-17A antibody (ab91649)
Key features and details
- Rabbit polyclonal to IL-17A
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-IL-17A antibody
See all IL-17A primary antibodies -
Description
Rabbit polyclonal to IL-17A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Mouse
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Immunogen
Synthetic peptide corresponding to Mouse IL-17A aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab104965) -
Positive control
- Recombinant Mouse IL-17 protein (ab50162) can be used as a positive control in WB. This antibody gave a positive signal in a mouse lymph node section.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P MouseAll applications RatApplication Abreviews Notes IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.Notes IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.Target
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Function
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
Tissue specificity
Restricted to activated memory T-cells. -
Sequence similarities
Belongs to the IL-17 family. -
Post-translational
modificationsFound both in glycosylated and nonglycosylated forms. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 16171 Mouse
- Entrez Gene: 301289 Rat
- SwissProt: Q62386 Mouse
- SwissProt: Q61453 Rat
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Alternative names
- CTLA 8 antibody
- CTLA-8 antibody
- CTLA8 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17A antibody (ab91649)
IHC image of IL-17A staining in mouse lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91649, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Datasheets and documents
References (6)
ab91649 has been referenced in 6 publications.
- Soley BDS et al. B1 and B2 kinin receptor blockade improves psoriasis-like disease. Br J Pharmacol N/A:N/A (2020). PubMed: 32335893
- Hsu PY et al. Cordycepin and a preparation fromCordyceps militarisinhibit malignant transformation and proliferation by decreasing EGFR and IL-17RA signaling in a murine oral cancer model. Oncotarget 8:93712-93728 (2017). PubMed: 29212184
- Cai S et al. IFN-? induction by neutrophil-derived IL-17A homodimer augments pulmonary antibacterial defense. Mucosal Immunol 9:718-29 (2016). PubMed: 26349661
- Chou TF et al. Tumour suppressor death-associated protein kinase targets cytoplasmic HIF-1a for Th17 suppression. Nat Commun 7:11904 (2016). WB, IHC ; Human . PubMed: 27312851
- Patras KA et al. Characterization of host immunity during persistent vaginal colonization by Group B Streptococcus. Mucosal Immunol 8:1339-48 (2015). IHC . PubMed: 25850655
- Usategui A et al. A profibrotic role for thymic stromal lymphopoietin in systemic sclerosis. Ann Rheum Dis N/A:N/A (2013). IHC ; Mouse . PubMed: 23413283
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17A antibody (ab91649)
IHC image of IL-17A staining in mouse lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91649, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
