Anti-IL-17A antibody (ab79056)
Key features and details
- Rabbit polyclonal to IL-17A
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-IL-17A antibody
See all IL-17A primary antibodies -
Description
Rabbit polyclonal to IL-17A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseIHC-P MouseHumanWB MouseHuman
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Immunogen
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Positive control
- Purchase matching WB positive control:Recombinant Human IL-17A protein
- IHC-P: lymphoid nodules
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General notes
The blocking peptide is available as ab190773
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17A antibody (ab79056)
Immunohistochemical analysis of paraffin-embedded Mouse Colon Tissue using ab79056 at 2 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunocytochemistry/ Immunofluorescence - Anti-IL-17A antibody (ab79056)Immunocytochemistry/Immunofluorescence analysis of A-20 (mouse reticulum sarcoma cell line) cells labelling IL-17A with purified ab79056 at 5 µg/mL. Cells were fixed with 4% paraformaldehyde. A goat anti-rabbit IgG (1/500) was used as the secondary antibody (green). Nuclei counterstained with DAPI (blue).
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Western blot - Anti-IL-17A antibody (ab79056)Lane 1 : Anti-IL-17A antibody (ab79056) at 0.5 µg/ml
Lane 2 : Anti-IL-17A antibody (ab79056) at 1 µg/ml
All lanes : A-20 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Additional bands at: 22 kDa, 28 kDa, 32 kDa. We are unsure as to the identity of these extra bands.The higher molecular weight bands seen in the Western blot may represent glycosylated IL-17A.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17A antibody (ab79056)Immunohistochemical analysis of paraffin-embedded Human Tonsil Tissue using ab79056 at 5 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17A antibody (ab79056) This image is courtesy of an Abreview submitted by Claudia Zizzadoroab79056 staining IL-17A in Dog lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with Tween-20 (0.05%) and blocked with 10% serum for 2 hours at 24°C; antigen retrieval was by heat mediation with citrate buffer (0.01M). Samples were incubated with primary antibody (1/100 in PBS +10% serum) for 14 hours at 4°C. A Biotin-conjugated Horse pan-specific anti-IgG polyclonal (1/20) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17A antibody (ab79056)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse thymus tissue staining IL-17A with ab79056 at 20µg/ml. Goat anti-rabbit IgG (1/500) was used as the secondary antibody (red).
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Western blot - Anti-IL-17A antibody (ab79056)All lanes : Anti-IL-17A antibody (ab79056)
Lane 1 : human thymus tissue lysate at 1 µg/ml
Lane 2 : human thymus tissue lysate at 2 µg/ml
Predicted band size: 18 kDa
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Western blot - Anti-IL-17A antibody (ab79056)Anti-IL-17A antibody (ab79056) at 1 µg/ml + Recombinant human IL-17 at 0.001 µg
Predicted band size: 18 kDa
