Anti-ADAM12 antibody (ab28747)
Key features and details
- Goat polyclonal to ADAM12
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ADAM12 antibody
See all ADAM12 primary antibodies -
Description
Goat polyclonal to ADAM12 -
Host species
Goat -
Specificity
This antibody is expected to recognise both the longer membrane bound form of human ADAM12 and the shorter soluble ADAM12 splice isoform. This antibody does not cross react with other ADAMS. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide:
AARPLPVSPARALC
, corresponding to N terminal amino acids 2-15 of ADAM12.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ADAM12 antibody (ab28747)Anti-ADAM12 antibody (ab28747) at 0.1 µg/ml + Human heart lysate (RIPA buffer, 30µg total protein per lane)
Predicted band size: 100 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Primary incubation was for 1 hour.
Detected using chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM12 antibody (ab28747)ab28747 at 3.8µg/ml staining ADAM12 in human prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval using citrate pH 6 was performed. AP staining used as detection method.
