Anti-ADAM12 antibody (ab223476)
Key features and details
- Goat polyclonal to ADAM12
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-ADAM12 antibody
See all ADAM12 primary antibodies -
Description
Goat polyclonal to ADAM12 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHumanPig
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Immunogen
Synthetic peptide corresponding to Human ADAM12 aa 200-300 (internal sequence) (Cysteine residue). (NP_003465.3).
Database link: O43184 -
Positive control
- WB: Human, mouse, rat and pig heart lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab223476 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ADAM12 antibody (ab223476)Anti-ADAM12 antibody (ab223476) at 0.1 µg/ml + Human heart lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 100 kDa
Observed band size: 100 kDaPrimary incubation was 1 hour.
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Western blot - Anti-ADAM12 antibody (ab223476)All lanes : Anti-ADAM12 antibody (ab223476) at 0.1 µg/ml
Lane 1 : Mouse heart lysate (in RIPA buffer)
Lane 2 : Rat heart lysate (in RIPA buffer)
Lane 3 : Pig heart lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 100 kDa
Observed band size: 60,90 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
