Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AMPK alpha 2 antibody [EP20772] - BSA and Azide free (ab225899)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP20772] to AMPK alpha 2 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-AMPK alpha 2 antibody [EP20772] - BSA and Azide free
    See all AMPK alpha 2 primary antibodies

  • Description

    Rabbit monoclonal [EP20772] to AMPK alpha 2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human skeletal muscle, fetal kidney and fetal liver tissue lysates.

  • General notes

    Ab225899 is the carrier-free version of ab214425. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab225899 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunoprecipitation - Anti-AMPK alpha 2 antibody [EP20772] - BSA and Azide free (ab225899)
    Immunoprecipitation - Anti-AMPK alpha 2 antibody [EP20772] - BSA and Azide free (ab225899)

    AMPK alpha 2 was immunoprecipitated from 0.35 mg of HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate with ab214425 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab214425 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293 whole cell lysate 10 µg (Input). 

    Lane 2: ab214425 IP in HEK-293 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214425 in HEK-293 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214425).

  • Western blot - Anti-AMPK alpha 2 antibody [EP20772] - BSA and Azide free (ab225899)
    Western blot - Anti-AMPK alpha 2 antibody [EP20772] - BSA and Azide free (ab225899)
    All lanes : Anti-AMPK alpha 2 antibody [EP20772] (ab214425) at 1/1000 dilution

    Lane 1 : Human skeletal muscle tissue lysate
    Lane 2 : Human fetal kidney tissue lysate
    Lane 3 : Human fetal liver tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 62 kDa



    Blocking/Dilution: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 5 seconds; Lane 3: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214425).

  • Anti-AMPK alpha 2 antibody [EP20772] - BSA and Azide free (ab225899)
    Anti-AMPK alpha 2 antibody [EP20772] - BSA and Azide free (ab225899)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-AMPK alpha 2 antibody [EP20772] - BSA and Azide free (ab225899)

© 2021 Astana Biomed Group LLP. All rights reserved.