Anti-AMPK alpha 2 antibody (ab97275)
Key features and details
- Rabbit polyclonal to AMPK alpha 2
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-AMPK alpha 2 antibody
See all AMPK alpha 2 primary antibodies -
Description
Rabbit polyclonal to AMPK alpha 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Pig
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 29 - 480 of Human AMPK alpha 2 (NP_006243).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AMPK alpha 2 antibody (ab97275)Anti-AMPK alpha 2 antibody (ab97275) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 62 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 2 antibody (ab97275)ab97275 at 1/200 dilution staining AMPK alpha 2 in paraformaldehyde-fixed A431 cells by immunofluorescence. The lower image shows ab97275 merged with a DNA probe. -
Western blot - Anti-AMPK alpha 2 antibody (ab97275)All lanes : Anti-AMPK alpha 2 antibody (ab97275) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 62 kDa
7.5% SDS PAGE -
Immunoprecipitation - Anti-AMPK alpha 2 antibody (ab97275)Lane 1: Load (1000 μg) HEK 293T lysate.
Lane 2: Immuniprecipitation with 2.5 μg preimmune serum.
Lane 3: Immunoprecipitation with 2.5 μg ab97275.
The blot was probed with ab97275 at a dilution of 1/1000.
