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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases

Anti-AMPK alpha 2 (phospho S345) antibody [EPMDAR6] (ab129081)

Price and availability

251 280 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-AMPK alpha 2 (phospho S345) antibody [EPMDAR6] (ab129081)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPMDAR6] to AMPK alpha 2 (phospho S345)
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-AMPK alpha 2 (phospho S345) antibody [EPMDAR6]
    See all AMPK alpha 2 primary antibodies
  • Description

    Rabbit monoclonal [EPMDAR6] to AMPK alpha 2 (phospho S345)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Phospho specific peptide corresponding to residues surrounding Ser345 of Human AMPK alpha 2 (UniProt ID: P54646).

  • Positive control

    • 293T cell lysates treated with Lambda Phosphatase
  • General notes

    This antibody was developed as part of a collaboration with Jiyong Liang from the MD Anderson Cancer Center.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPMDAR6
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Images

  • Western blot - Anti-AMPK alpha 2 (phospho S345) antibody [EPMDAR6] (ab129081)
    Western blot - Anti-AMPK alpha 2 (phospho S345) antibody [EPMDAR6] (ab129081)
    All lanes : Anti-AMPK alpha 2 (phospho S345) antibody [EPMDAR6] (ab129081) at 1/1000 dilution

    Lane 1 : 293T cell lysate, untreated
    Lane 2 : 293T cell lysate treated with Lambda Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

  • Anti-AMPK alpha 2 (phospho S345) antibody [EPMDAR6] (ab129081)
    Anti-AMPK alpha 2 (phospho S345) antibody [EPMDAR6] (ab129081)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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