Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STK33 antibody [EPR15343] - BSA and Azide free (ab251446)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15343] to STK33 - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-STK33 antibody [EPR15343] - BSA and Azide free
    See all STK33 primary antibodies

  • Description

    Rabbit monoclonal [EPR15343] to STK33 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251446 is the carrier-free version of ab206296. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251446 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-STK33 antibody [EPR15343] - BSA and Azide free (ab251446)
    Western blot - Anti-STK33 antibody [EPR15343] - BSA and Azide free (ab251446)
    All lanes : Anti-STK33 antibody [EPR15343] (ab206296) at 1/1000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : HEK293 (Human epithelial cell line from embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 58, 50 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab206296, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Can recognize two isoforms. The predicted MW are 58KDa and 50KDa, respectively.

  • Western blot - Anti-STK33 antibody [EPR15343] - BSA and Azide free (ab251446)
    Western blot - Anti-STK33 antibody [EPR15343] - BSA and Azide free (ab251446)
    Anti-STK33 antibody [EPR15343] (ab206296) at 1/1000 dilution + Human testis tissue lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 58, 50 kDa
    Observed band size: 58, 50 kDa


    Exposure time: 1 minute


    This data was developed using ab206296, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Can recognize two isoforms. The predicted MW are 58KDa and 50KDa, respectively.

  • Western blot - Anti-STK33 antibody [EPR15343] - BSA and Azide free (ab251446)
    Western blot - Anti-STK33 antibody [EPR15343] - BSA and Azide free (ab251446)
    All lanes : Anti-STK33 antibody [EPR15343] (ab206296) at 1/1000 dilution

    Lane 1 : Human fetal kidney tissue lysate
    Lane 2 : Human fetal spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 58, 50 kDa
    Observed band size: 58 kDa why is the actual band size different from the predicted?



    This data was developed using ab206296, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 10 seconds; Lane 2: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK33 antibody [EPR15343] - BSA and Azide free (ab251446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK33 antibody [EPR15343] - BSA and Azide free (ab251446)
    This data was developed using ab206296, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human testis tissue labeling STK33 with ab206296 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on human testis tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK33 antibody [EPR15343] - BSA and Azide free (ab251446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK33 antibody [EPR15343] - BSA and Azide free (ab251446)
    This data was developed using ab206296, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human fetal cardiac tissue labeling STK33 with ab206296 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on human fetal cardiac muscle tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-STK33 antibody [EPR15343] - BSA and Azide free (ab251446)
    Immunocytochemistry - Anti-STK33 antibody [EPR15343] - BSA and Azide free (ab251446)
    This data was developed using ab206296, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling STK33 with ab206296 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).Cytoplasmic and nuclear staining on Jurkat cell line is observed.The nuclear counterstain is DAPI (blue).Tubulin is detected with anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:-
    -ve control 1 - ab206296 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2. - anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
  • Anti-STK33 antibody [EPR15343] - BSA and Azide free (ab251446)
    Anti-STK33 antibody [EPR15343] - BSA and Azide free (ab251446)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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