Anti-Casein Kinase 1 gamma 2 antibody (ab230045)
Key features and details
- Rabbit polyclonal to Casein Kinase 1 gamma 2
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Casein Kinase 1 gamma 2 antibody
See all Casein Kinase 1 gamma 2 primary antibodies -
Description
Rabbit polyclonal to Casein Kinase 1 gamma 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Casein Kinase 1 gamma 2 aa 300 to the C-terminus.
Database link: P78368 -
Positive control
- WB: Mouse gonadal tissue; HeLa whole cell lysate. IHC-P: Human testis tissue and human breast cancer tissue. IP: HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Casein Kinase 1 gamma 2 antibody (ab230045)All lanes : Anti-Casein Kinase 1 gamma 2 antibody (ab230045) at 1/1000 dilution
Lane 1 : Mouse gonadal tissue lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
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Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody (ab230045)Casein Kinase 1 gamma 2 was immunoprecipitated from 500 µg HeLa epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 µg of ab230045. Western blot was performed from the immunoprecipitate using ab230045 at 1/1000 dilution.
Lane 1: 1 µg of Rabbit monoclonal IgG instead of ab230045 in HeLa whole cell lysate.
Lane 2: ab230045 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg (input).For western blotting, an HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used the secondary antibody (1/50,000).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 1 gamma 2 antibody (ab230045)Paraffin-embedded human breast cancer tissue stained for Casein Kinase 1 gamma 2 using ab230045 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 1 gamma 2 antibody (ab230045)Paraffin-embedded human testis tissue stained for Casien Kinase 1 gamma 2 using ab230045 at 1/100 dilution in immunohistochemical analysis.
