Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-2N Tau antibody [EPR21723] (ab218316)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21723] to 2N Tau
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-2N Tau antibody [EPR21723]
    See all 2N Tau primary antibodies

  • Description

    Rabbit monoclonal [EPR21723] to 2N Tau

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged human 2N3R Tau recombinant protein (aa1-410); His-tagged human 2N4R Tau recombinant protein (aa1-441); Human and mouse hippocampus and brain lysates; Rat hippocampus lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-2N Tau antibody [EPR21723] (ab218316)
    Western blot - Anti-2N Tau antibody [EPR21723] (ab218316)
    All lanes : Anti-2N Tau antibody [EPR21723] (ab218316) at 1/1000 dilution

    Lane 1 : Human hippocampus lysate
    Lane 2 : Human brain lysate
    Lane 3 : Human stomach lysate
    Lane 4 : Mouse hippocampus lysate
    Lane 5 : Mouse brain lysate
    Lane 6 : Mouse stomach lysate
    Lane 7 : Rat hippocampus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
    Lanes 4-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 78 kDa
    Observed band size: 36-75 kDa
    why is the actual band size different from the predicted?



    Exposure time : Lanes 1-3: 3 minutes; Lanes 4-6: 59 seconds; Lane 7: 3 minutes.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Negative control: Mouse stomach, human stomach. PMID:8752131; PMID:11727254.

  • Western blot - Anti-2N Tau antibody [EPR21723] (ab218316)
    Western blot - Anti-2N Tau antibody [EPR21723] (ab218316)
    All lanes : Anti-2N Tau antibody [EPR21723] (ab218316) at 1/1000 dilution

    Lane 1 : His-tagged human 2N3R Tau recombinant protein (aa1-410), 10 ng
    Lane 2 : His-tagged human 2N4R Tau recombinant protein (aa1-441), 10 ng
    Lane 3 : His-tagged human 0N3R Tau recombinant protein (aa1-352), 10 ng
    Lane 4 : His-tagged human 0N4R Tau recombinant protein (aa1-383), 10 ng
    Lane 5 : His-tagged human 1N3R Tau recombinant protein (aa1-381), 10 ng
    Lane 6 : His-tagged human 1N4R Tau recombinant protein (aa1-412), 10 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 78 kDa



    Exposure time : Lane 1: 1 second; Lanes 2-8: 3 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This antibody specifically recognizes 2N3R and 2N4R tau recombinant proteins. The lower bands maybe degraded tau fragments (PMID:28045602).

  • Anti-2N Tau antibody [EPR21723] (ab218316)
    Anti-2N Tau antibody [EPR21723] (ab218316)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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