Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-2N Tau antibody [EPR21723] - BSA and Azide free (ab242345)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21723] to 2N Tau - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-2N Tau antibody [EPR21723] - BSA and Azide free
    See all 2N Tau primary antibodies

  • Description

    Rabbit monoclonal [EPR21723] to 2N Tau - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human and mouse hippocampus and brain lysates; Rat hippocampus lysate.

  • General notes

    Ab242345 is the carrier-free version of ab218316. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab242345 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-2N Tau antibody [EPR21723] - BSA and Azide free (ab242345)
    Western blot - Anti-2N Tau antibody [EPR21723] - BSA and Azide free (ab242345)
    All lanes : Anti-2N Tau antibody [EPR21723] (ab218316) at 1/1000 dilution

    Lane 1 : Human hippocampus lysate
    Lane 2 : Human brain lysate
    Lane 3 : Human stomach lysate
    Lane 4 : Mouse hippocampus lysate
    Lane 5 : Mouse brain lysate
    Lane 6 : Mouse stomach lysate
    Lane 7 : Rat hippocampus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
    Lanes 4-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 78 kDa
    Observed band size: 36-75 kDa
    why is the actual band size different from the predicted?



    Exposure time : Lanes 1-3: 3 minutes; Lanes 4-6: 59 seconds; Lane 7: 3 minutes.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Negative control: Mouse stomach, human stomach. PMID:8752131; PMID:11727254.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218316).

     

  • Anti-2N Tau antibody [EPR21723] - BSA and Azide free (ab242345)
    Anti-2N Tau antibody [EPR21723] - BSA and Azide free (ab242345)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-2N Tau antibody [EPR21723] - BSA and Azide free (ab242345)

© 2021 Astana Biomed Group LLP. All rights reserved.