Anti-AMPK alpha 2 antibody [EP20772] (ab214425)
![Anti-AMPK alpha 2 antibody [EP20772] (ab214425)](https://www.abcam.com/ps/products/214/ab214425/Images/ab214425-284744-AMPK-alpha-2WB1.jpg "Anti-AMPK alpha 2 antibody [EP20772] (ab214425)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP20772] to AMPK alpha 2
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-AMPK alpha 2 antibody [EP20772]
See all AMPK alpha 2 primary antibodies -
Description
Rabbit monoclonal [EP20772] to AMPK alpha 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293, HepG2 and HeLa whole cell lysates; Human skeletal muscle, fetal kidney and fetal liver tissue lysates. IP: HEK-293 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP20772 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AMPK alpha 2 antibody [EP20772] (ab214425)All lanes : Anti-AMPK alpha 2 antibody [EP20772] (ab214425) at 1/5000 dilution
Lane 1 : His-tagged human AMPK alpha 1 recombinant protein (aa1-559)
Lane 2 : His-tagged human AMPK alpha 2 recombinant protein (aa1-552)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 1 secondBlocking/Dilution: 5% NFDM/TBST.
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![Western blot - Anti-AMPK alpha 2 antibody [EP20772] (ab214425)](https://www.abcam.com/ps/products/214/ab214425/Images/ab214425-284745-AMPK-alpha-2WB2.jpg)
Western blot - Anti-AMPK alpha 2 antibody [EP20772] (ab214425)All lanes : Anti-AMPK alpha 2 antibody [EP20772] (ab214425) at 1/1000 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 62 kDa
Observed band size: 62 kDaBlocking/Dilution: 5% NFDM/TBST.
Exposure times: Lanes 1-2: 30 seconds; Lane 3: 3 minutes.
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![Western blot - Anti-AMPK alpha 2 antibody [EP20772] (ab214425)](https://www.abcam.com/ps/products/214/ab214425/Images/ab214425-284746-AMPK-alpha-2WB3.jpg)
Western blot - Anti-AMPK alpha 2 antibody [EP20772] (ab214425)All lanes : Anti-AMPK alpha 2 antibody [EP20772] (ab214425) at 1/1000 dilution
Lane 1 : Human skeletal muscle tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lane 3 : Human fetal liver tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 62 kDa
Observed band size: 62 kDaBlocking/Dilution: 5% NFDM/TBST.
Exposure times: Lanes 1-2: 5 seconds; Lane 3: 3 minutes.
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![Immunoprecipitation - Anti-AMPK alpha 2 antibody [EP20772] (ab214425)](https://www.abcam.com/ps/products/214/ab214425/Images/ab214425-284747-AMPK-alpha-2IP.jpg)
Immunoprecipitation - Anti-AMPK alpha 2 antibody [EP20772] (ab214425)AMPK alpha 2 was immunoprecipitated from 0.35 mg of HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate with ab214425 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab214425 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Lane 1: HEK-293 whole cell lysate 10 µg (Input).
Lane 2: ab214425 IP in HEK-293 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214425 in HEK-293 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Anti-AMPK alpha 2 antibody [EP20772] (ab214425)](https://www.abcam.com/ps/products/214/ab214425/Images/ab214425-5-benefits-of-recombinant-antibodies.png)
Anti-AMPK alpha 2 antibody [EP20772] (ab214425)
