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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-DRP1 antibody [OTI4F6] (ab156951)

Anti-DRP1 antibody [OTI4F6] (ab156951)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [OTI4F6] to DRP1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Dog, Human, African green monkey
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-DRP1 antibody [OTI4F6]
    See all DRP1 primary antibodies
  • Description

    Mouse monoclonal [OTI4F6] to DRP1
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-736 of Human DRP1 produced in HEK293T cells (NP_036192).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HEK293T cells transfected with pCMV6-ENTRY DRP1 cDNA; HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF7 cell extracts; Human lung carcinoma, ovary adenocarcinoma, pancreas, pancreas carcinoma and endometrium tissues.
  • General notes

    Dilute in PBS (pH7.3) before use.

    The clone number has been updated from 4F6 to OTI4F6, both clone numbers name the same clone.

    This product was changed from ascites to tissue culture supernatant on 6th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 1% BSA, 48% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from TCS
  • Clonality

    Monoclonal
  • Clone number

    OTI4F6
  • Isotype

    IgG2b
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Other
    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Western blot - Anti-DRP1 antibody [OTI4F6] (ab156951)
    Western blot - Anti-DRP1 antibody [OTI4F6] (ab156951)
    All lanes : Anti-DRP1 antibody [OTI4F6] (ab156951) at 1/200 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY DRP1 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 82 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone RC200418
  • Western blot - Anti-DRP1 antibody [OTI4F6] (ab156951)
    Western blot - Anti-DRP1 antibody [OTI4F6] (ab156951)
    All lanes : Anti-DRP1 antibody [OTI4F6] (ab156951) at 1/200 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 82 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone RC200418
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [OTI4F6] (ab156951)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [OTI4F6] (ab156951)
    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling DRP1 with ab156951 at 1/150 dilution
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [OTI4F6] (ab156951)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [OTI4F6] (ab156951)
    Immunohistochemical analysis of paraffin-embedded Human ovary adenocarcinoma tissue labeling DRP1 with ab156951 at 1/150 dilution
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [OTI4F6] (ab156951)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [OTI4F6] (ab156951)
    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling DRP1 with ab156951 at 1/150 dilution
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [OTI4F6] (ab156951)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [OTI4F6] (ab156951)
    Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma tissue labeling DRP1 with ab156951 at 1/150 dilution
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [OTI4F6] (ab156951)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [OTI4F6] (ab156951)
    Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling DRP1 with ab156951 at 1/150 dilution

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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