Anti-DRP1 antibody [OTI4F6] (ab156951)
Key features and details
- Mouse monoclonal [OTI4F6] to DRP1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Dog, Human, African green monkey
- Isotype: IgG2b
Overview
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Product name
Anti-DRP1 antibody [OTI4F6]
See all DRP1 primary antibodies -
Description
Mouse monoclonal [OTI4F6] to DRP1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Recombinant full length protein, corresponding to amino acids 1-736 of Human DRP1 produced in HEK293T cells (NP_036192).
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Positive control
- HEK293T cells transfected with pCMV6-ENTRY DRP1 cDNA; HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF7 cell extracts; Human lung carcinoma, ovary adenocarcinoma, pancreas, pancreas carcinoma and endometrium tissues.
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General notes
Dilute in PBS (pH7.3) before use.
The clone number has been updated from 4F6 to OTI4F6, both clone numbers name the same clone.
This product was changed from ascites to tissue culture supernatant on 6th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, 48% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
OTI4F6 -
Isotype
IgG2b -
Research areas
Images
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All lanes : Anti-DRP1 antibody [OTI4F6] (ab156951) at 1/200 dilution
Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY DRP1 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 82 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone RC200418 -
All lanes : Anti-DRP1 antibody [OTI4F6] (ab156951) at 1/200 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HeLa cell extract
Lane 3 : SVT2 cell extract
Lane 4 : A549 cell extract
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat cell extract
Lane 7 : MDCK cell extract
Lane 8 : PC12 cell extract
Lane 9 : MCF7 cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 82 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone RC200418 -
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling DRP1 with ab156951 at 1/150 dilution
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Immunohistochemical analysis of paraffin-embedded Human ovary adenocarcinoma tissue labeling DRP1 with ab156951 at 1/150 dilution
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Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling DRP1 with ab156951 at 1/150 dilution
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Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma tissue labeling DRP1 with ab156951 at 1/150 dilution
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Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling DRP1 with ab156951 at 1/150 dilution