Antibodies, Proteins, Kits and Reagents for Life Science

Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20311] to ITLN1+ITLN2 - BSA and Azide free
Suitable for: WB, IHC-Fr, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free
See all ITLN1+ITLN2 primary antibodies
Description

Rabbit monoclonal [EPR20311] to ITLN1+ITLN2 - BSA and Azide free
Host species

Rabbit
Specificity

ITLN1 mRNA expression in stomach has been reported [PMID: 19549487]. Another report [PMID: 22768319] shows no staining on stomach at protein level. In our hands this antibody showed weak cytoplasmic staining on stomach samples.
Tested applications

Suitable for: WB, IHC-Fr, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab251518 is the carrier-free version of ab210483. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251518 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR20311
Isotype

IgG
Research areas

Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)
Immunohistochemistry (Frozen sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

This data was developed using ab210483, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on goblet cells of mouse colon is observed [PMID: 22768319] [PMID: 19549487]. The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

This data was developed using ab210483, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on goblet cells of human colon is observed [PMID: 22768319] [PMID: 19549487]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

This data was developed using ab210483, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on goblet cells of mouse colon is observed [PMID: 22768319] [PMID: 19549487]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

This data was developed using ab210483, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on goblet cells of rat colon is observed [PMID: 22768319] [PMID: 19549487]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

All lanes : Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483) at 1/5000 dilution

Lane 1 : Human colon lysate
Lane 2 : Human fetal spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 35 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?

Exposure time: 5 seconds

This data was developed using ab210483, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Spleen is a negative control (PMID: 11181563).
Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

All lanes : Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483) at 1/5000 dilution

Lane 1 : Mouse colon lysate
Lane 2 : Rat small intestine lysate
Lane 3 : Mouse small intestine lysate
Lane 4 : Rat colon lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?

This data was developed using ab210483, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST. Exposure times: Lanes 1-2: 1 minute; Lanes 3-4: 30 seconds.

The observed molecular weight is consistent with the literature (PMID: 11313366, 20628387).
Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483) at 1/5000 dilution + Human small intestine lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?

Exposure time: 1 second

This data was developed using ab210483, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

The observed molecular weight is consistent with the literature (PMID: 11313366 and 20628387).
Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com