Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20311] to ITLN1+ITLN2 - BSA and Azide free
  • Suitable for: WB, IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free
    See all ITLN1+ITLN2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20311] to ITLN1+ITLN2 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    ITLN1 mRNA expression in stomach has been reported [PMID: 19549487]. Another report [PMID: 22768319] shows no staining on stomach at protein level. In our hands this antibody showed weak cytoplasmic staining on stomach samples.

  • Tested applications

    Suitable for: WB, IHC-Fr, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251518 is the carrier-free version of ab210483. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251518 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)
    Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)
  • Immunohistochemistry (Frozen sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)
    Immunohistochemistry (Frozen sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

    This data was developed using ab210483, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on goblet cells of mouse colon is observed [PMID: 22768319] [PMID: 19549487]. The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

    This data was developed using ab210483, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on goblet cells of human colon is observed [PMID: 22768319] [PMID: 19549487]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)
    This data was developed using ab210483, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on goblet cells of mouse colon is observed [PMID: 22768319] [PMID: 19549487]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)
    This data was developed using ab210483, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on goblet cells of rat colon is observed [PMID: 22768319] [PMID: 19549487]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)
    Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)
    All lanes : Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483) at 1/5000 dilution

    Lane 1 : Human colon lysate
    Lane 2 : Human fetal spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    This data was developed using ab210483, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Spleen is a negative control (PMID: 11181563).

  • Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)
    Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)
    All lanes : Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483) at 1/5000 dilution

    Lane 1 : Mouse colon lysate
    Lane 2 : Rat small intestine lysate
    Lane 3 : Mouse small intestine lysate
    Lane 4 : Rat colon lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?



    This data was developed using ab210483, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST. Exposure times: Lanes 1-2: 1 minute; Lanes 3-4: 30 seconds.

    The observed molecular weight is consistent with the literature (PMID: 11313366, 20628387).

  • Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)
    Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)
    Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483) at 1/5000 dilution + Human small intestine lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    This data was developed using ab210483, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The observed molecular weight is consistent with the literature (PMID: 11313366 and 20628387).

  • Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)
    Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

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