Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LLGL1 antibody [EPR18899] - BSA and Azide free (ab250640)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18899] to LLGL1 - BSA and Azide free
  • Suitable for: ICC, IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-LLGL1 antibody [EPR18899] - BSA and Azide free
    See all LLGL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18899] to LLGL1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 53% identity (67% positives) with LLGL2. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested applications

    Suitable for: ICC, IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250640 is the carrier-free version of ab183021. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250640 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-LLGL1 antibody [EPR18899] - BSA and Azide free (ab250640)
    Western blot - Anti-LLGL1 antibody [EPR18899] - BSA and Azide free (ab250640)
    All lanes : Anti-LLGL1 antibody [EPR18899] (ab183021) at 1/5000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : SW480 cell lysate
    Lane 4 : A459 cell lysate
    Lane 5 : Caco-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 115 kDa
    Observed band size: 115 kDa



    This data was developed using ab183021, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-LLGL1 antibody [EPR18899] - BSA and Azide free (ab250640)
    Western blot - Anti-LLGL1 antibody [EPR18899] - BSA and Azide free (ab250640)
    All lanes : Anti-LLGL1 antibody [EPR18899] (ab183021) at 1/2000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 115 kDa
    Observed band size: 115 kDa



    This data was developed using ab183021, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 3 minutes; Lane 3: 15 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LLGL1 antibody [EPR18899] - BSA and Azide free (ab250640)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LLGL1 antibody [EPR18899] - BSA and Azide free (ab250640)
    This data was developed using ab183021, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling LLGL1 with ab183021 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human kidney is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LLGL1 antibody [EPR18899] - BSA and Azide free (ab250640)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LLGL1 antibody [EPR18899] - BSA and Azide free (ab250640)
    This data was developed using ab183021, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling LLGL1 with ab183021 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human stomach is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-LLGL1 antibody [EPR18899] - BSA and Azide free (ab250640)
    Immunocytochemistry - Anti-LLGL1 antibody [EPR18899] - BSA and Azide free (ab250640)
    This data was developed using ab183021, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling LLGL1 with ab183021 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [EPR18899]-Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).The negative controls are as follows:
    -ve control 1: ab183021 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
  • Immunocytochemistry - Anti-LLGL1 antibody [EPR18899] - BSA and Azide free (ab250640)
    Immunocytochemistry - Anti-LLGL1 antibody [EPR18899] - BSA and Azide free (ab250640)
    This data was developed using ab183021, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (Human colorectal adenocarcinoma cell line) cells labeling LLGL1 with ab183021 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SW480 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [EPR18899]-Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).The negative controls are as follows:
    -ve control 1: ab183021 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
  • Immunoprecipitation - Anti-LLGL1 antibody [EPR18899] - BSA and Azide free (ab250640)
    Immunoprecipitation - Anti-LLGL1 antibody [EPR18899] - BSA and Azide free (ab250640)
    This data was developed using ab183021, the same antibody clone in a different buffer formulation.LLGL1was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab183021 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab183021 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
    Lane 1: HepG2 whole cell lysate 10µg (Input).
    Lane 2: ab183021 IP in HepG2 whole cell lysate.
    Lane 3: Rabbit IgG, monoclonal [EPR18899] - Isotype Control (ab172730) instead of ab183021 in HepG2 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds.
  • Anti-LLGL1 antibody [EPR18899] - BSA and Azide free (ab250640)
    Anti-LLGL1 antibody [EPR18899] - BSA and Azide free (ab250640)

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