Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Scavenging Receptor SRB2 antibody [EPR12081] - BSA and Azide free (ab251221)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12081] to Scavenging Receptor SRB2 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Scavenging Receptor SRB2 antibody [EPR12081] - BSA and Azide free
    See all Scavenging Receptor SRB2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12081] to Scavenging Receptor SRB2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251221 is the carrier-free version of ab196651. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251221 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - BSA and Azide free (ab251221)
    Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - BSA and Azide free (ab251221)
    All lanes : Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651) at 1/20000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab196651, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID: 21389126, 22272359, and 23635510).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - BSA and Azide free (ab251221)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - BSA and Azide free (ab251221)
    This data was developed using ab196651, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Scavenging Receptor SRB2 with ab196651 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on human liver tissue is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
  • Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - BSA and Azide free (ab251221)
    Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - BSA and Azide free (ab251221)
    Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651) at 1/20000 dilution + Human fetal kidney tissue lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    This data was developed using ab196651, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID: 21389126; 22272359; 23635510).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - BSA and Azide free (ab251221)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - BSA and Azide free (ab251221)
    This data was developed using ab196651, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Scavenging Receptor SRB2 with ab196651 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on mouse liver tissue is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
  • Flow Cytometry - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - BSA and Azide free (ab251221)
    Flow Cytometry - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - BSA and Azide free (ab251221)
    This data was developed using ab196651, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling Scavenging Receptor SRB2 with ab196651 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
  • Anti-Scavenging Receptor SRB2 antibody [EPR12081] - BSA and Azide free (ab251221)
    Anti-Scavenging Receptor SRB2 antibody [EPR12081] - BSA and Azide free (ab251221)

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