Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-AMPK gamma 1 antibody [Y307] (ab32382)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [Y307] to AMPK gamma 1
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-AMPK gamma 1 antibody [Y307]
See all AMPK gamma 1 primary antibodies
Description

Rabbit monoclonal [Y307] to AMPK gamma 1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human AMPK gamma 1 aa 1-100. The exact sequence is proprietary.
Epitope

ab32382 reacts with an epitope located in the N terminal region of AMPK gamma 1.
Positive control
- Jurkat cell lysate and HeLa cell lysate, human skeletal muscles
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

Y307
Isotype

IgG
Research areas

Western blot - Anti-AMPK gamma 1 antibody [Y307] (ab32382)

All lanes : Anti-AMPK gamma 1 antibody [Y307] (ab32382) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PRKAG1 (AMPK gamma 1) knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 38 kDa

Lanes 1 - 3: Merged signal (red and green). Green - ab32382 observed at 38 kDa. Red - loading control, Mouse anti Vinculin, observed at 125 kDa.

ab32382 was shown to recognize AMPK gamma 1 in wild-type HAP1 cells as signal was lost at the expected MW in PRKAG1 (AMPK gamma 1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PRKAG1 (AMPK gamma 1) knockout samples were subjected to SDS-PAGE. Ab32382 and Mouse anti-Vinculin (ab9484 loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-AMPK gamma 1 antibody [Y307] (ab32382)

All lanes : Anti-AMPK gamma 1 antibody [Y307] (ab32382) at 1/2000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate

Predicted band size: 38 kDa
Observed band size: 37 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK gamma 1 antibody [Y307] (ab32382)

This image shows paraffin embedded human skeletal muscle stained with 1/250 ab23282.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-AMPK gamma 1 antibody [Y307] (ab32382) This image is courtesy of an anonymous Abreview

All lanes : Anti-AMPK gamma 1 antibody [Y307] (ab32382) at 1/2000 dilution

Lane 1 : HeLa whole cell lystate - Control Si
Lane 2 : HeLa whole cell lystate - treated with AMPK gamma 1 siRNA A for 36 hours
Lane 3 : HeLa whole cell lystate - treated with AMPK gamma 1 siRNA B for 36 hours

Secondary
All lanes : HRP-conjugated Donkey polyclonal to rabbit IgG at 1/3000 dilution

Predicted band size: 38 kDa

See Abreview
Anti-AMPK gamma 1 antibody [Y307] (ab32382)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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