Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AMPK gamma 1 antibody [Y307] (ab32382)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y307] to AMPK gamma 1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-AMPK gamma 1 antibody [Y307]
    See all AMPK gamma 1 primary antibodies

  • Description

    Rabbit monoclonal [Y307] to AMPK gamma 1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human AMPK gamma 1 aa 1-100. The exact sequence is proprietary.

  • Epitope

    ab32382 reacts with an epitope located in the N terminal region of AMPK gamma 1.

  • Positive control

    • Jurkat cell lysate and HeLa cell lysate, human skeletal muscles

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-AMPK gamma 1 antibody [Y307] (ab32382)
    Western blot - Anti-AMPK gamma 1 antibody [Y307] (ab32382)
    All lanes : Anti-AMPK gamma 1 antibody [Y307] (ab32382) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : PRKAG1 (AMPK gamma 1) knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 38 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab32382 observed at 38 kDa. Red - loading control, Mouse anti Vinculin, observed at 125 kDa.

    ab32382 was shown to recognize AMPK gamma 1 in wild-type HAP1 cells as signal was lost at the expected MW in PRKAG1 (AMPK gamma 1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PRKAG1 (AMPK gamma 1) knockout samples were subjected to SDS-PAGE. Ab32382 and Mouse anti-Vinculin (ab9484 loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-AMPK gamma 1 antibody [Y307] (ab32382)
    Western blot - Anti-AMPK gamma 1 antibody [Y307] (ab32382)
    All lanes : Anti-AMPK gamma 1 antibody [Y307] (ab32382) at 1/2000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate

    Predicted band size: 38 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK gamma 1 antibody [Y307] (ab32382)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK gamma 1 antibody [Y307] (ab32382)

    This image shows paraffin embedded human skeletal muscle stained with 1/250 ab23282.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-AMPK gamma 1 antibody [Y307] (ab32382)
    Western blot - Anti-AMPK gamma 1 antibody [Y307] (ab32382) This image is courtesy of an anonymous Abreview
    All lanes : Anti-AMPK gamma 1 antibody [Y307] (ab32382) at 1/2000 dilution

    Lane 1 : HeLa whole cell lystate - Control Si
    Lane 2 : HeLa whole cell lystate - treated with AMPK gamma 1 siRNA A for 36 hours
    Lane 3 : HeLa whole cell lystate - treated with AMPK gamma 1 siRNA B for 36 hours

    Secondary
    All lanes : HRP-conjugated Donkey polyclonal to rabbit IgG at 1/3000 dilution

    Predicted band size: 38 kDa

    See Abreview

  • Anti-AMPK gamma 1 antibody [Y307] (ab32382)
    Anti-AMPK gamma 1 antibody [Y307] (ab32382)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-AMPK gamma 1 antibody [Y307] (ab32382)

© 2021 Astana Biomed Group LLP. All rights reserved.