Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 16, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4955] to EHD1
Suitable for: WB, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-EHD1 antibody [EPR4955]
See all EHD1 primary antibodies
Description

Rabbit monoclonal [EPR4955] to EHD1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HepG2, HeLa and JAR cell lysates; HeLa cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4955
Isotype

IgG
Research areas

Flow Cytometry - Anti-EHD1 antibody [EPR4955] (ab109747)

Overlay histogram showing HeLa cells stained with ab109747 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109747, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - Anti-EHD1 antibody [EPR4955] (ab109747)

All lanes : Anti-EHD1 antibody [EPR4955] (ab109747) at 1/10000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : JAR cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 61 kDa
Immunocytochemistry/ Immunofluorescence - Anti-EHD1 antibody [EPR4955] (ab109747)

ab109747, at 1/100 dilution, staining EHD1 in HeLa cells by Immunofluorescence.
Anti-EHD1 antibody [EPR4955] (ab109747)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com