Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EHD1 antibody [EPR4954] (ab109311)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4954] to EHD1
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-EHD1 antibody [EPR4954]
    See all EHD1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4954] to EHD1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, Human testis, SW480, SK-OV-3, Human brain, HeLa, Mouse brain, and Rat brain lysates. IHC-P: human thyroid carcinoma, kidney and tonsil, mouse lung, and rat lung tissues. IP: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling EHD1 with purified ab109311 at 1/120 dilution (2.85 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)
    Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)
    All lanes : Anti-EHD1 antibody [EPR4954] (ab109311) at 1/10000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 61 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-EHD1 antibody [EPR4954] (ab109311)
    Immunoprecipitation - Anti-EHD1 antibody [EPR4954] (ab109311)

    ab109311 (purified ) at 1/30 dilution (2ug) immunoprecipitating EHD1 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab109311 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109311 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue sections labeling EHD1 with purified ab109311 at 1/1600 dilution (0.21 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)
    Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)
    Anti-EHD1 antibody [EPR4954] (ab109311) at 1/10000 dilution (Purified) + Human brain lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 61 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)
    Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)
    All lanes : Anti-EHD1 antibody [EPR4954] (ab109311) at 1/1000 dilution ((unpurified))

    Lane 1 : HepG2 cell lysates
    Lane 2 : Human testis lysates
    Lane 3 : SW480 cell lysates
    Lane 4 : SK-OV-3 cell lysates
    Lane 5 : HeLa cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 61 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling EHD1 with purified ab109311 at 1/1600 dilution (0.21 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)

    Immunohistochemical analysis of EHD1 in paraffin embedded Human kidney tissue, using unpurified ab109311 at a 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)

    Immunohistochemical analysis of EHD1 in paraffin embedded Human tonsil tissue, using unpurified ab109311 at a 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-EHD1 antibody [EPR4954] (ab109311)
    Anti-EHD1 antibody [EPR4954] (ab109311)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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