Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4954] to EHD1
Suitable for: WB, IP, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-EHD1 antibody [EPR4954]
See all EHD1 primary antibodies
Description

Rabbit monoclonal [EPR4954] to EHD1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HepG2, Human testis, SW480, SK-OV-3, Human brain, HeLa, Mouse brain, and Rat brain lysates. IHC-P: human thyroid carcinoma, kidney and tonsil, mouse lung, and rat lung tissues. IP: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4954
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling EHD1 with purified ab109311 at 1/120 dilution (2.85 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)

All lanes : Anti-EHD1 antibody [EPR4954] (ab109311) at 1/10000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 60 kDa
why is the actual band size different from the predicted?
Immunoprecipitation - Anti-EHD1 antibody [EPR4954] (ab109311)

ab109311 (purified ) at 1/30 dilution (2ug) immunoprecipitating EHD1 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab109311 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109311 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue sections labeling EHD1 with purified ab109311 at 1/1600 dilution (0.21 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)

Anti-EHD1 antibody [EPR4954] (ab109311) at 1/10000 dilution (Purified) + Human brain lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)

All lanes : Anti-EHD1 antibody [EPR4954] (ab109311) at 1/1000 dilution ((unpurified))

Lane 1 : HepG2 cell lysates
Lane 2 : Human testis lysates
Lane 3 : SW480 cell lysates
Lane 4 : SK-OV-3 cell lysates
Lane 5 : HeLa cell lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling EHD1 with purified ab109311 at 1/1600 dilution (0.21 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)

Immunohistochemical analysis of EHD1 in paraffin embedded Human kidney tissue, using unpurified ab109311 at a 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)

Immunohistochemical analysis of EHD1 in paraffin embedded Human tonsil tissue, using unpurified ab109311 at a 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-EHD1 antibody [EPR4954] (ab109311)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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