FITC Anti-Ly6g antibody [RB6-8C5] (ab25024)
Key features and details
- FITC Rat monoclonal [RB6-8C5] to Ly6g
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Overview
-
Product name
FITC Anti-Ly6g antibody [RB6-8C5]
See all Ly6g primary antibodies -
Description
FITC Rat monoclonal [RB6-8C5] to Ly6g -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab25024 recognises mouse Mouse Ly-6G. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
-
Positive control
- mouse spleen cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
RB6-8C5 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
-
Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling Ly6g with ab25024 0.5µg (right panel), Rat IgG2b (FITC) isotype control at 0.5µg (left panel).
-
BALB/c bone marrow cells were stained with either rat IgG2b-FITC (as an isotype control) or rat anti-mouse Ly-6G-FITC (ab25024) at a concentration of 1ug/106 cells. Large cells were then gated and analyzed on a flow cytometer.
-
Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling Ly6g with ab25024 at 0.5µg.
-
ab25024 at a 1/100 dilution detecting Ly6g in LPS activated human monocytes by Flow Cytometry.Gating strategy was for alive cells.
-
ab25024 at a 1/100 dilution detecting Ly6g (Gr1) in murine blood cells by Flow Cytometry.
Gating strategy was for monocytes. -
ab25024 staining Ly6g in Cynomolgus Monkey whole blood by Flow Cytometry. Red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide and fixed in paraformaldehyde. The sample was incubated with the primary antibody (1/10 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
Gating Strategy: Live Monocytes.