Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1901(2)] to PKC alpha (phospho S657)
Suitable for: Dot blot, IHC-P, WB
Reacts with: Rat, Human

Product name

Anti-PKC alpha (phospho S657) antibody [EPR1901(2)]
See all PKC alpha primary antibodies
Description

Rabbit monoclonal [EPR1901(2)] to PKC alpha (phospho S657)
Host species

Rabbit
Specificity

ab180848 only detects PKC alpha phosphorylated at serine 657.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Rat

Human

See all applications and species data
Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PKC alpha aa 650 to the C-terminus (phospho S657) (Cysteine residue). The exact sequence is proprietary.
Database link: P17252
Positive control
- Jurkat cell lysate. Human heart and stomach tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR1901(2)
Isotype

IgG
Research areas

Western blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848)

All lanes : Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848) at 1/10000 dilution

Lane 1 : Untreated C6 (rat glioma) whole cell lysate
Lane 2 : C6 (rat glioma) treated with 80nM Phorbol-12-myristate-13-acetate, whole cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 77 kDa
Additional bands at: 77 kDa. We are unsure as to the identity of these extra bands.

Blocking and diluting buffer: 5% NFDM/TBST
Dot Blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848)

Dot blot analysis of PKC alpha (pS657) phospho peptide (Lane 1) and PKC alpha non-phospho peptide (Lane 2) using ab180848 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.

Blocking and Diluting buffer and concentration: 5% NFDM /TBST.

Exposure time: 10 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848)

ab180848 staining PKC alpha (phospho S657) in human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1: PBS in place of primary antibody.
Western blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848)

All lanes : Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848) at 1/10000 dilution

Lane 1 : Untreated Jurkat (human acute T cell leukemia) whole cell lysate
Lane 2 : Jurkat (human acute T cell leukemia) treatrd with 200ng/ml phorbol-12,13-dibutyrate for 45 minutes, whole cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 77 kDa
Additional bands at: 77 kDa. We are unsure as to the identity of these extra bands.

Blocking and diluting buffer: 5% NFDM/TBST
Western blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848)

All lanes : Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848)

Lane 1 : Jurkat cell lysate
Lane 2 : Lysate from Jurkat cells treated with phorbol-12,13-dibutyrate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 77 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human heart tissue labeling PKC alpha (phospho S657) with ab180848.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human stomach tissue labeling PKC alpha (phospho S657) with ab180848.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com